AUTHOR=Wei Ling-Ling , Chen Tian-Tian , Luo Bi-Yun , Qiu Gao-Feng 

TITLE=Evidences for Red Pigment Concentrating Hormone (RPCH) and Beta-Pigment Dispersing Hormone (β-PDH) Inducing Oocyte Meiotic Maturation in the Chinese Mitten Crab, Eriocheir sinensis

JOURNAL=Frontiers in Endocrinology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2021.802768

DOI=10.3389/fendo.2021.802768

ISSN=1664-2392

ABSTRACT=<p>Red pigment concentrating hormone (RPCH) and pigment dispersing hormone (PDH) are crustacean neuropeptides involved in broad physiological processes including body color changes, circadian rhythm, and ovarian growth. In this study, the full-length cDNA of <italic>RPCH</italic> and <italic>PDH</italic> were identified from the brain of the Chinese mitten crab <italic>Eriocheir sinensis</italic>. The deduced RPCH and PDH mature peptides shared identical sequence to the adipokinetic hormone/RPCH peptides family and the β-PDH isoforms and were designated as Es-RPCH and Es-β-PDH, respectively. <italic>Es-RPCH</italic> and <italic>Es-β-PDH</italic> transcripts were distributed in the brain and eyestalks. The positive signals of <italic>Es-RPCH</italic> and <italic>Es-β-PDH</italic> were localized in the neuronal clusters 6, 8, 9, 10, and 17 of the brain as revealed by <italic>in situ</italic> hybridization. The expression level of <italic>Es-RPCH</italic> and <italic>Es-β-PDH</italic> mRNA in nervous tissues were all significantly increased at vitellogenic stage, and then decreased at the final meiotic maturation stage. The administrated with synthesized Es-RPCH peptide results in germinal vesicles shift toward the plasma membrane in vitellogenic oocyte, and significant decrease of the gonad-somatic index (GSI) and mean oocyte diameter as well as the expression of vitellogenin mRNA at 30 days post injection <italic>in vivo</italic>. Similar results were also found when injection of the Es-β-PDH peptide. <italic>In vitro</italic> culture demonstrated that Es-RPCH and Es-β-PDH induced germinal vesicle breakdown of the late vitellogenic oocytes. Comparative ovarian transcriptome analysis indicated that some reproduction/meiosis-related genes such as cdc2 kinase, cyclin B, 5-HT-R and retinoid-X receptor were significantly upregulated in response to Es-RPCH and Es-β-PDH treatments. Taken together, these results provided the evidence for the inductive effect of <italic>Es-RPCH</italic> and <italic>Es-β-PDH</italic> on the oocyte meiotic maturation in <italic>E. sinensis</italic>.</p>