AUTHOR=Yang Meng-Ting , Chang Wei-Hung , Kuo Tien-Fen , Shen Ming-Yi , Yang Chu-Wen , Tien Yin-Jing , Lai Bun-Yueh , Chen Yet-Ran , Chang Yi-Cheng , Yang Wen-Chin TITLE=Identification of Novel Biomarkers for Pre-diabetic Diagnosis Using a Combinational Approach JOURNAL=Frontiers in Endocrinology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2021.641336 DOI=10.3389/fendo.2021.641336 ISSN=1664-2392 ABSTRACT=
Reliable protein markers for pre-diabetes in humans are not clinically available. In order to identify novel and reliable protein markers for pre-diabetes in humans, healthy volunteers and patients diagnosed with pre-diabetes and stroke were recruited for blood collection. Blood samples were collected from healthy and pre-diabetic subjects 12 h after fasting. BMI was calculated from body weight and height. Fasting blood glucose (FBG), glycated hemoglobin (HbA1C), triglyceride (TG), total cholesterol, high-density lipoprotein, low-density lipoprotein (LDL), insulin and albumin were assayed by automated clinical laboratory methods. We used a quantitative proteomics approach to identify 1074 proteins from the sera of pre-diabetic and healthy subjects. Among them, 500 proteins were then selected using Mascot analysis scores. Further, 70 out of 500 proteins were selected