AUTHOR=Han Meifang , Ma Ke , Wang Xiaojing , Yan Weiming , Wang Hongwu , You Jie , Wang Qiuxia , Chen Huilong , Guo Wei , Chen Tao , Ning Qin , Luo Xiaoping 

TITLE=Immunological Characteristics in Type 2 Diabetes Mellitus Among COVID-19 Patients

JOURNAL=Frontiers in Endocrinology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2021.596518

DOI=10.3389/fendo.2021.596518

ISSN=1664-2392

ABSTRACT=<sec><title>Clinical Trial Registration</title><p><uri xlink:href="http://www.ClinicalTrials.gov" xmlns:xlink="http://www.w3.org/1999/xlink">www.ClinicalTrials.gov</uri>, identifier: NCT04365634.</p></sec><sec><title>Context</title><p>Diabetes mellitus was associated with increased severity and mortality of disease in COVID-19 pneumonia. So far the effect of type 2 diabetes (T2DM) or hyperglycemia on the immune system among COVID-19 disease has remained unclear.</p></sec><sec><title>Objective</title><p>We aim to explore the clinical and immunological features of type 2 diabetes mellitus (T2DM) among COVID-19 patients.</p></sec><sec><title>Design and Methods</title><p>In this retrospective study, the clinical and immunological characteristics of 306 hospitalized confirmed COVID-19 patients (including 129 diabetic and 177 non-diabetic patients) were analyzed. The serum concentrations of laboratory parameters including cytokines and numbers of immune cells were measured and compared between diabetic and non-diabetic groups.</p></sec><sec><title>Results</title><p>Compared with non-diabetic group, diabetic cases more frequently had lymphopenia and hyperglycemia, with higher levels of urea nitrogen, myoglobin, D-dimer and ferritin. Diabetic cases indicated the obviously elevated mortality and the higher levels of cytokines IL‐2R, IL‐6, IL‐8, IL‐10, and TNF‐α, as well as the distinctly reduced Th1/Th2 cytokines ratios compared with non-diabetic cases. The longitudinal assays showed that compared to that at week 1, the levels of IL-6 and IL-8 were significantly elevated at week 2 after admission in non-survivors of diabetic cases, whereas there were greatly reductions from week 1 to week 2 in survivors of diabetic cases. Compared with survival diabetic patients, non-survival diabetic cases displayed distinct higher serum concentrations of IL-2R, IL-6, IL-8, IL-10, TNF‐α, and lower Th1/Th2 cytokines ratios at week 2. Samples from a subset of participants were evaluated by flow cytometry for the immune cells. The counts of peripheral total T lymphocytes, CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and NK cells were markedly lower in diabetic cases than in non-diabetic cases. The non-survivors showed the markedly declined counts of CD8<sup>+</sup> T cells and NK cells than survivors.</p></sec><sec><title>Conclusion</title><p>The elevated cytokines, imbalance of Th1/Th2 cytokines ratios and reduced of peripheral numbers of CD8<sup>+</sup> T cells and NK cells might contribute to the pathogenic mechanisms of high mortality of COVID-19 patients with T2DM.</p></sec>