AUTHOR=Ogawa Satoshi , Ramadasan Priveena Nair , Anthonysamy Rachel , Parhar Ishwar S. 

TITLE=Sexual Dimorphic Distribution of Hypothalamic Tachykinin1 Cells and Their Innervations to GnRH Neurons in the Zebrafish

JOURNAL=Frontiers in Endocrinology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2020.534343

DOI=10.3389/fendo.2020.534343

ISSN=1664-2392

ABSTRACT=<p>Substance P (SP) and neurokinin A (NKA), encoded by <italic>TAC1</italic>/<italic>Tac1</italic> gene are members of the tachykinin family, which exert their neuromodulatory roles in vertebrate reproduction. In mammals, SP and NKA have been shown to regulate gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) secretion <italic>via</italic> kisspeptin neurons. On the other hand, the role of SP/NKA in the regulation of reproduction in non-mammalian vertebrates is not well known. In the present study, we first localized expression of <italic>tac1</italic> mRNA in the brain of male and female zebrafish, <italic>Danio rerio</italic>. Next, using an antibody against zebrafish tachykinin1 (Tac1), we examined the neural association of SP/NKA neural processes with GnRH3 neurons, and with kisspeptin (<italic>kiss2</italic>) neurons, in the brains of male and female zebrafish. <italic>In situ</italic> hybridization showed an apparent male-dominant <italic>tac1</italic> expression in the ventral telencephalic area, the anterior and posterior parts of the parvocellular preoptic nucleus, and the suprachiasmatic nucleus. On the other hand, there was female-dominant <italic>tac1</italic> expression in the ventral periventricular hypothalamus. Confocal images of double-labeled zebrafish Tac1 and GnRH3 showed associations between Tac1-immunoreactive processes and GnRH3 neurons in the ventral telencephalic area. In contrast, there was no apparent proximity of Tac1 processes to <italic>kiss2</italic> mRNA-expressing neurons in the hypothalamus. Lastly, to elucidate possible direct action of SP/NKA on GnRH3 or Kiss2 neurons, expression of SP/NKA receptor, <italic>tacr1a</italic> mRNA was examined in regions containing GnRH3 or Kiss2 neurons by <italic>in situ</italic> hybridization. Expression of <italic>tacr1a</italic> mRNA was seen in several brain regions including the olfactory bulb, preoptic area and hypothalamus, where GnRH3 and Kiss2 cells are present. These results suggest that unlike in mammals, Tac1 may be involved in male reproductive functions <italic>via</italic> direct action on GnRH3 neurons but independent of kisspeptin in the zebrafish.</p>