AUTHOR=Di Benedetto Adriana , Posa Francesca , Marazzi Mario , Kalemaj Zamira , Grassi Roberta , Lo Muzio Lorenzo , Comite Mariasevera Di , Cavalcanti-Adam Elisabetta Ada , Grassi Felice Roberto , Mori Giorgio 

TITLE=Osteogenic and Chondrogenic Potential of the Supramolecular Aggregate T-LysYal®

JOURNAL=Frontiers in Endocrinology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2020.00285

DOI=10.3389/fendo.2020.00285

ISSN=1664-2392

ABSTRACT=<p>Hard tissue regeneration represents a challenge for the Regenerative Medicine and Mesenchymal stem cells (MSCs) could be a successful therapeutic strategy. T-LysYal® (T-Lys), a new derivative of Hyaluronic Acid (HA) possessing a superior stability, has already been proved efficient in repairing corneal epithelial cells damaged by dry conditions <italic>in vitro</italic>. We investigated the regenerative potential of T-Lys in the hard tissues bone and cartilage. We have previously demonstrated that cells isolated from the tooth germ, Dental Bud Stem Cells (DBSCs), differentiate into osteoblast-like cells, representing a promising source of MSCs for bone regeneration. Herewith, we show that T-Lys treatment stimulates the expression of typical osteoblastic markers, such as <italic>Runx-2, Collagen I</italic> (<italic>Col1</italic>) and Alkaline Phosphatase (ALP), determining a higher production of mineralized matrix nodules. In addition, we found that T-Lys treatment positively affects α<sub>V</sub>β<sub>3</sub> integrin expression, key integrin in the osteoblastic commitment, leading to the formation of focal adhesions (FAs). The efficacy of T-Lys was also tested on chondrogenic differentiation starting from human articular chondrocytes (HACs) resulting in an increase of differentiation markers and cell number.</p>