AUTHOR=Sun Da-Guang , Tian Shi , Zhang Lu , Hu Yi , Guan Chun-Yi , Ma Xu , Xia Hong-Fei 

TITLE=The miRNA-29b Is Downregulated in Placenta During Gestational Diabetes Mellitus and May Alter Placenta Development by Regulating Trophoblast Migration and Invasion Through a HIF3A-Dependent Mechanism

JOURNAL=Frontiers in Endocrinology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2020.00169

DOI=10.3389/fendo.2020.00169

ISSN=1664-2392

ABSTRACT=<p>Gestational diabetes mellitus (GDM) is a disease that changes the function of microvascular of placenta. MicroRNA (miRNA) expression in placenta may contribute to the pathogenesis of GDM. Here, we evaluate the role and function of <italic>miR-29b</italic> in the development of GDM. This study discovered that <italic>miR-29b</italic> expression was lower in placentas derived from patients with GDM than that in control placentas. <italic>MiR-29b</italic> over-expression inhibited cell growth and migration, and <italic>miR-29b</italic> knockdown promoted cell migration. Then we predicted and confirmed that <italic>HIF3A</italic> was a direct target of <italic>miR-29b</italic> with two specific binding sites at the recognition sequences of <italic>miR-29b</italic> in 3′-UTR of <italic>HIF3A</italic> mRNA, which was negatively correlated with <italic>miR-29b</italic> expression level. The up-regulation of <italic>HIF3A</italic> partially antagonized the inhibitory effect of <italic>miR-29b</italic> over-expression on cell growth and migration. The enhancement of cell migration induced by <italic>miR-29b</italic> knockdown was attenuated by down-regulating <italic>HIF3A</italic>. These results imply that down-regulation of <italic>miR-29b</italic> may be related with the development of GDM partially via increasing the expression of <italic>HIF3A</italic>, which may provide a new insight for the mechanism of GDM.</p>