AUTHOR=Cunningham Thomas Keith , Allgar Victoria , Dargham Soha R. , Kilpatrick Eric , Sathyapalan Thozhukat , Maguiness Stephen , Mokhtar Rudin Haira R. , Abdul Ghani Nour M. , Latiff Aishah , Atkin Stephen L. 

TITLE=Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment

JOURNAL=Frontiers in Endocrinology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2019.00013

DOI=10.3389/fendo.2019.00013

ISSN=1664-2392

ABSTRACT=<p><bold>Objective:</bold> The relationship between fertilization rates and 1,25-dihydroxyvitamin D (1,25(OH)<sub>2</sub>D<sub>3</sub>), 25-hydroxyvitamin D2 (25(OH)D<sub>2</sub>), 25-hydroxyvitamin D3 (25(OH)D<sub>3</sub>), 24,25-dihydroxyvitamin D (24,25(OH)<sub>2</sub>D<sub>3</sub>), and 25-hydroxy-3epi-Vitamin D3 (3epi25(OH)D<sub>3</sub>) concentrations in age and weight matched women with and without PCOS was studied.</p><p><bold>Methods:</bold> Fifty nine non-obese women, 29 with PCOS, and 30 non-PCOS undergoing IVF, matched for age and weight were included. Serum vitamin D metabolites were taken the menstrual cycle prior to commencing controlled ovarian hyperstimulation.</p><p><bold>Results:</bold> Vitamin D metabolites did not differ between PCOS and controls; however, 25(OH)D<sub>3</sub> correlated with embryo fertilization rates in PCOS patients alone (<italic>p</italic> = 0.03). For all subjects, 3epi25(OH)D<sub>3</sub> correlated with fertilization rate (<italic>p</italic> &lt; 0.04) and negatively with HOMA-IR (<italic>p</italic> &lt; 0.02); 25(OH)D<sub>2</sub> correlated with cleavage rate, G3D3 and blastocyst (<italic>p</italic> &lt; 0.05; <italic>p</italic> &lt; 0.009; <italic>p</italic> &lt; 0.002, respectively). 24,25(OH)<sub>2</sub>D<sub>3</sub> correlated with AMH, antral follicle count, eggs retrieved and top quality embryos (G3D3) (<italic>p</italic> &lt; 0.03; <italic>p</italic> &lt; 0.003; <italic>p</italic> &lt; 0.009; <italic>p</italic> &lt; 0.002, respectively), and negatively with HOMA-IR (<italic>p</italic> &lt; 0.01). 1,25(OH)<sub>2</sub>D<sub>3</sub> did not correlate with any of the metabolic or embryo parameters. In slim PCOS, 25(OH)D<sub>3</sub> correlated with increased fertilization rates in PCOS, but other vitamin D parameters did not differ to matched controls.</p><p><bold>Conclusion:</bold> 3epi25(OH)D<sub>3</sub>, 25(OH)D<sub>2</sub>, and 24,25(OH)<sub>2</sub>D<sub>3</sub>, but not 1,25(OH)<sub>2</sub>D<sub>3</sub>, were associated with embryo parameters suggesting that vitamin D metabolites other than 1,25(OH)<sub>2</sub>D<sub>3</sub> are important in fertility.</p>