AUTHOR=Blanco Ayelén M. , Velasco Cristina , Bertucci Juan I. , Soengas José L. , Unniappan Suraj 

TITLE=Nesfatin-1 Regulates Feeding, Glucosensing and Lipid Metabolism in Rainbow Trout

JOURNAL=Frontiers in Endocrinology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2018.00484

DOI=10.3389/fendo.2018.00484

ISSN=1664-2392

ABSTRACT=<p>Nesfatin-1 is an 82 amino acid peptide that has been involved in a wide variety of physiological functions in both mammals and fish. This study aimed to elucidate the role of nesfatin-1 on rainbow trout food intake, and its putative effects on glucose and fatty acid sensing systems. Intracerebroventricular administration of 25 ng/g nesfatin-1 resulted in a significant inhibition of appetite, likely mediated by the activation of central POMC and CART. Nesfatin-1 stimulated the glucosensing machinery (changes in <italic>sglt1, g6pase, gsase</italic>, and <italic>gnat3</italic> mRNA expression) in the hindbrain and hypothalamus. Central fatty acid sensing mechanisms were unaltered by nesfatin-1, but this peptide altered the expression of mRNAs encoding factors regulating lipid metabolism (<italic>fat/cd36, acly, mcd, fas, lpl, ppar</italic>α, and <italic>ppar</italic>γ), suggesting that nesfatin-1 promotes lipid accumulation in neurons. In the liver, intracerebroventricular nesfatin-1 treatment resulted in decreased capacity for glucose use and lipogenesis, and increased the potential of fatty acid oxidation. Altogether, the present results demonstrate that nesfatin-1 is involved in the homeostatic regulation of food intake and metabolism in fish.</p>