Samantha Zwicker ^* Clara Sánchez Clemens Lukasser

• Hoja Nueva, Puerto Maldonado, Peru

This study provides novel insights into the ecology of the margay (Leopardus wiedii), focusing on its occupancy, density, and activity patterns in the Madre de Dios region, Peru, by utilizing both arboreal and terrestrial camera traps. Conducted across 10 km2, the research achieved 47 detections, utilizing semi-arboreal, lower canopy cameras for the first time to capture margay activities. Occupancy models revealed a mean occupancy probability of 53.82% and a detection probability of 6.57%. Among the environmental covariates, diameter at breast height (DBH) was identified as a significant predictor, negatively impacting occupancy, suggesting margays favor areas with smaller tree diameters. Contrary to expectations, the Normalized Difference Vegetation Index (NDVI) did not significantly influence occupancy. Tree density exhibited a positive, though non-significant, association with margay presence. Spatial capture-recapture (SECR) models estimated a margay density of 71.46 individuals per 100 km², with significant sex-based differences in spatial behavior. Males demonstrated larger home ranges (approximately 13.50 km²) compared to females (approximately 3.79 km²). Activity pattern analyses indicated primary nocturnal behavior with peaks at midnight to 3 am, 5 am, and 6 pm. Temporal overlap analysis revealed a low overlap coefficient with jaguarundis (Dhat1 = 0.21) and a higher overlap with ocelots (Dhat4 = 0.79), reflecting intricate interspecies dynamics. Our findings highlight important ecological aspects of margay behavior, including habitat preferences, nocturnal activity patterns, and interspecies interactions, which were effectively captured through the combined use of terrestrial and arboreal camera traps. The study emphasizes the importance of habitat preservation and the development of conservation strategies tailored to the ecological needs of margays, potentially influencing global practices for the management and protection of lesser-studied semi-arboreal wild cats.