AUTHOR=Koteska Diana , Wang Hui , Wagner-Döbler Irene , Schulz Stefan 

TITLE=Outer membrane vesicles of Dinoroseobacter shibae transport a volatile aldehyde

JOURNAL=Frontiers in Ecology and Evolution

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2023.1102159

DOI=10.3389/fevo.2023.1102159

ISSN=2296-701X

ABSTRACT=<p>Outer Membrane Vesicles (OMVs) of the Gram-negative marine bacterium <italic>Dinoroseobacter shibae</italic>, a member of the Roseobacteraceae, were investigated for the presence of volatile organic compounds (VOCs). Extracts of vesicles were analyzed by gas chromatography/mass spectrometry (GC/MS). In these analyses the short fatty acid (<italic>Z</italic>)-5-dodecenoic acid (<bold>1</bold>) and the related, more volatile aldehyde (<italic>Z</italic>)-5-dodecenal (<bold>8</bold>) were identified as VOCs of the OMVs. The aldehyde <bold>8</bold> has not yet been reported before from bacteria. Due to their possible function as signaling molecules, both compounds were tested for Quorum Sensing (QS) inhibition in a bioassay against the QS sensor strain <italic>Pseudomonas putida</italic> F117 (pKRC12) responsive to long-chain <italic>N</italic>-acylhomoserine lactones, the effectors of the sensor. Both compounds showed QS inhibitory activity. The potential function of VOCs in OMVs which has not been observed previously is discussed.</p>