AUTHOR=Lin Aiqing , Feng Jiang , Kanwal Jagmeet S. TITLE=Geographic Variation in Social Vocalizations of the Great Himalayan Leaf-Nosed Bat, Hipposideros armiger: Acoustic Overflow Across Population Boundaries JOURNAL=Frontiers in Ecology and Evolution VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2022.948324 DOI=10.3389/fevo.2022.948324 ISSN=2296-701X ABSTRACT=

Bat populations employ rich vocal repertoires for social communication in addition to emitting sound pulses for echolocation. Acoustic parameters of echolocation pulses can vary with the context in which they are emitted, and also with the individual and across populations as a whole. The acoustic parameters of social vocalizations, or “calls”, also vary with the individual and context, but not much is known about their variation across populations at different geographic locations. Here, we leveraged the detailed acoustic classification of social vocalizations available for the Great Himalayan leaf-nosed bat, Hipposideros armiger, to examine geographic variation in five commonly emitted simple syllable types. We hypothesized that individuals within geographically dispersed populations communicate using spectrographically similar constructs or “syllable types”. We also examined whether call syllables vary discordantly with the correlation pattern observed for echolocation pulses across those same geographic regions. Furthermore, we postulated that the acoustic boundaries of a syllable type are not uniquely constrained to its variation within a particular population of the same subspecies. To test our hypotheses, we obtained recordings of social calls of H. a. armiger from nine locations within the oriental region. These locations were consolidated into five geographic regions based on previously established region-specific differences in the peak frequency of echolocation pulses. A multivariate cluster analysis established that unlike echolocation pulses, syllable types exhibit a relatively large variance. Analysis of this variance showed significant differences in Least Squares Means estimates, establishing significant population-level differences in the multiparametric means of individual syllable types across geographic regions. Multivariate discriminant analysis confirmed the presence of region-specific centroids for different syllable constructs, but also showed a large overlap of their multiparametric boundaries across geographic regions. We propose that despite differences in the population-specific core construct of a syllable type, bats maximize acoustic variation across individuals within a population irrespective of its overflow and overlap with other populations.