AUTHOR=Cui Hu , Wang Lixia , Zhang Jian 

TITLE=Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures

JOURNAL=Frontiers in Ecology and Evolution

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2022.1019494

DOI=10.3389/fevo.2022.1019494

ISSN=2296-701X

ABSTRACT=<p>There are a considerable number of discussions aimed at analyzing microbial communities and their functions during the composting process. However, microbial succession under copper (Cu) and tetracycline (TCH)-stressed conditions has received less attention. Thus, this work analyzed the bacterial and fungal structures with high-throughput sequencing in Cu/TCH-amended composting (Cu: 0, 100, and 500 mg/kg; TCH: 0, 50, and 300 mg/kg), and the dominating controls on microbial diversity were identified using redundancy analysis (RDA) and structural equation models (SEMs). Low-concentration Cu increased the peak temperature (57°C) at the thermophilic phase. Composting phase-derived changes in bacterial and fungal communities were significant, while Cu and TCH showed a remarkable influence on fungi but not on bacteria. Cu and TCH inhibited <italic>Firmicutes'</italic> activity while promoting <italic>Actinobacteriota</italic> growth. Low-concentration Cu and TCH had a negative effect on <italic>Basidiomycota</italic> in the thermophilic phase and a positive influence on <italic>Chytridiomycota</italic> in the mature phase. TOC and TN were primary controls on the changes in microbial communities. NH<inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mrow></mml:mrow><mml:mrow><mml:mn>4</mml:mn></mml:mrow><mml:mrow><mml:mo>+</mml:mo></mml:mrow></mml:msubsup></mml:math></inline-formula>-N and NO<inline-formula><mml:math id="M2" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mrow></mml:mrow><mml:mrow><mml:mn>3</mml:mn></mml:mrow><mml:mrow><mml:mo>-</mml:mo></mml:mrow></mml:msubsup></mml:math></inline-formula>-N were more beneficial to fungi with a contribution proportion of 42.13 and 16.85%, respectively. These findings could provide theoretical guidance for the directional research on microbial inoculants.</p>