AUTHOR=Chan Nathan , Shen Zeli , Mannion Anthony , Kurnick Susanna , Popescu Ioana S. , Burton Frederic J. , Calle Paul P. , Fox James G. 

TITLE=Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi)

JOURNAL=Frontiers in Ecology and Evolution

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2021.676682

DOI=10.3389/fevo.2021.676682

ISSN=2296-701X

ABSTRACT=<p>Blue iguanas (<italic>Cyclura lewisi</italic>) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel <italic>Helicobacter</italic> species GCBI1 was detected in sick and dead iguanas. In the current study, fecal and cloacal swab samples were obtained from 25 iguanas. Through molecular and microbiological techniques, a novel <italic>Helicobacter</italic> species was cultured from feces and characterized, for whom we propose the name <italic>Helicobacter cyclurae</italic>. This novel helicobacter had a prevalence of 56% by PCR and 20% by culture in samples analyzed. The type strain MIT 16-1353 was catalase, oxidase, and gamma-glutamyl transpeptidase positive. By electron microscopy, <italic>H. cyclurae</italic> has a curved rod morphology and a single sheathed polar flagellum. Phylogenetic analysis using 16S rRNA, <italic>gyr</italic>B, and <italic>hsp</italic>60 indicated that these strains were most closely related to <italic>Helicobacter</italic> sp. 12502256-12 previously isolated from lizards. <italic>H. cyclurae</italic> has a 1.91-Mb genome with a GC content of 33.37%. There were 1,969 genes with four notable virulence genes: high temperature requirement-A protein-secreted serine protease, gamma-glutamyl transpeptidase, fibronectin/fibrinogen binding protein, and neutrophil-activating protein. Whole-genome phylogeny, average nucleotide identity, and digital DNA–DNA hybridization analysis confirmed that <italic>H. cyclurae</italic> is a novel species, and the first helicobacter cultured and characterized from blue iguanas.</p>