AUTHOR=Zhong Haixia , Zhang Fuchun , Zhou Xiaoming , Pan Mingqi , Xu Juan , Hao Jingzhe , Han Shouan , Mei Chuang , Xian He , Wang Min , Ji Jiahui , Shi Wei , Wu Xinyu TITLE=Genome-Wide Identification of Sequence Variations and SSR Marker Development in the Munake Grape Cultivar JOURNAL=Frontiers in Ecology and Evolution VOLUME=9 YEAR=2021 URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2021.664835 DOI=10.3389/fevo.2021.664835 ISSN=2296-701X ABSTRACT=

The Munake grape cultivar produces uniquely flavored and high-quality fruits. Despite the numerous beneficial agronomic traits of Munake, there are few genetic resources available for this cultivar. To address this knowledge gap, the entire genome was sequenced using whole-genome sequencing approaches and compared with a Vitis vinifera L. reference genome. This study describes more than 3 million single nucleotide polymorphism (SNP), 300,000 insertion and deletion (InDel), 14,000 structural variation (SV), and 80,000 simple sequence repeat (SSR) markers (one SSR per 4.23 kb), as well as their primers. Among the SSRs, 44 SSR primer pairs were randomly selected and validated by polymerase chain reaction (PCR), allowing discrimination between the different Munake cultivar genotypes. The genetic data provided allow a deeper understanding of Munake cultivar genomic sequence and contribute to better knowledge of the genetic basis behind its key agronomic traits.