AUTHOR=Martínez David , Hernández Carolina , Muñoz Marina , Armesto Yulieth , Cuervo Andres , Ramírez Juan David 

TITLE=Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

JOURNAL=Frontiers in Ecology and Evolution

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2020.602190

DOI=10.3389/fevo.2020.602190

ISSN=2296-701X

ABSTRACT=<p>The identification of vector species and their natural infection with arboviruses results in important data for the control of their transmission. However, for the eastern region of Colombia, this information is limited. Therefore, this study morphologically and molecularly identified species of the genus <italic>Aedes</italic> and the detection of arboviruses (Dengue, Chikungunya, Zika, and Mayaro) in female mosquitoes (individually) present in three municipalities (Saravena, Arauquita, and Tame) by amplifying the genetic material using RT-PCR (reverse transcriptase polymerase chain reaction) in the department of Arauca, eastern Colombia. Inconsistencies between morphological and molecular identification were detected in 13 individuals with <italic>Aedes albopictus</italic> initially determined as <italic>Aedes aegypti</italic> based on morphology (<italic>n</italic> = 13). Molecular identification showed the simultaneous presence of <italic>A. aegypti</italic> (n = 111) and <italic>A. albopictus</italic> (<italic>n</italic> = 58) in the urban municipalities of Saravena and Arauquita. These individuals were naturally infected with Dengue virus type 1 (DENV-1) and Chikungunya virus (CHIKV). The most frequent arbovirus was DENV-1 with an infection rate of 40.7% (11/27) for <italic>A. aegypti</italic> and 39.7% (23/58) for <italic>A. albopictus</italic>, which was followed by CHIKV with an infection rate of 1.8% for <italic>A. aegypti</italic> (2/111) and 6.9% for <italic>A. albopictus</italic> (4/58). Additionally, a mixed infection of DENV-1 and CHIKV was obtained in 4.5% of <italic>A. aegypti</italic> (5/111). Zika virus (ZIKV) and Mayaro virus (MAYV) infections were not detected. This study found that barcoding (fragment gene COI) is a successful method for identifying <italic>Aedes</italic> species. Additionally, we recommend the individual processing of insects as a more accurate strategy for arboviruses detection since the infection rate is obtained and co-infection between DENV-1 and CHIKV is also possible.</p>