AUTHOR=Subalusky Amanda L. , Dutton Christopher L. , Rosi Emma J. , Puth Linda M. , Post David M. TITLE=A River of Bones: Wildebeest Skeletons Leave a Legacy of Mass Mortality in the Mara River, Kenya JOURNAL=Frontiers in Ecology and Evolution VOLUME=8 YEAR=2020 URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2020.00031 DOI=10.3389/fevo.2020.00031 ISSN=2296-701X ABSTRACT=

Animal carcasses can provide important resources for a suite of consumers, and bones may provide a largely overlooked component of this resource, as they contain a large proportion of the phosphorus (P) in a carcass and they can persist for decades to millennia. We synthesized several datasets from our research in the Mara River, in which annual mass drownings of wildebeest (Connochaetes taurinus) contribute 2.2 × 105 kg of bones per year, to examine the ecological role that bone could play in this river ecosystem and to prioritize research questions on the role of bones in aquatic ecosystems in general. We measured bone stoichiometry and used in-stream litterbags to measure bone decomposition rate, both of which varied by bone type. Decomposition occurs as a two-stage process, with 15% of the mass being relatively labile and decomposing in 80–120 days and the remaining recalcitrant portion decomposing over > 80 years, leading to an estimated standing stock of 5.1 × 106 kg bones in the river. We used mesocosm experiments to measure leaching rates from bones. Leachate from fresh bones was an order of magnitude higher in inorganic nitrogen (N) than P; however, aged bones from the river leached much more P than N, which stimulated primary production. Biofilms growing on bones had five times greater chlorophyll a and two times greater organic matter than those growing on rocks, although algal composition was not significantly different between the two substrates. Biofilms growing on bones also differed from biofilms on rocks in carbon (C) and N stable isotope signature. Mixing models suggest that biofilms on bones account for 19% of macroinvertebrate and 24% of fish tissues in the Mara River, even months after carcasses were present. In combination, these findings suggest that bones may influence nutrient cycling, ecosystem function, and food webs in the Mara River, potentially on decadal time scales. Bones may also be important in other aquatic ecosystems, and mass extirpations of large land mammals may have led to a loss of this resource. Large animal bones may play a unique role in ecosystems via their slow release of limiting nutrients.