AUTHOR=Lee Ze Hong , Tatsumi Yoshitaka , Ichihashi Yasunori , Suzuki Takamasa , Shibata Arisa , Shirasu Ken , Yamaguchi Nobutoshi , Ito Toshiro 

TITLE=CRABS CLAW and SUPERMAN Coordinate Hormone-, Stress-, and Metabolic-Related Gene Expression During Arabidopsis Stamen Development

JOURNAL=Frontiers in Ecology and Evolution

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2019.00437

DOI=10.3389/fevo.2019.00437

ISSN=2296-701X

ABSTRACT=<p>The appropriate timing of the termination of floral meristem activity (FM determinacy) determines the number of floral organs. In <italic>Arabidopsis</italic>, two transcription factors, CRABS CRAW (CRC) and SUPERMAN (SUP), play key roles in FM determinacy. CRC belongs to the YABBY transcription factor family, whose members contain a zinc finger and a helix-loop-helix domain. The <italic>crc</italic> mutation causes the formation of unfused carpels and leads to an increase in carpel number in sensitized backgrounds. The <italic>SUP</italic> gene encodes a C2H2-type zinc-finger protein, and <italic>sup</italic> mutants produce extra carpels and stamens. However, the genetic interaction between <italic>CRC</italic> and <italic>SUP</italic> is not fully understood. Here, we show that these two transcription factors regulate multiple common downstream genes during stamen development. The <italic>crc sup</italic> double mutant had significantly more stamens and carpels than the parental lines and an enlarged floral meristem. Transcriptome data have implicated several cytokinin- and auxin-related genes as well as stress- and metabolic-related genes to function downstream of CRC and SUP during stamen development. The regulation of common downstream genes of CRC and SUP might contribute to the initiation of an appropriate number of stamens and to subsequent growth and development.</p>