AUTHOR=Fischer Benjamin M. C. , Frentress Jay , Manzoni Stefano , Cousins Sara A. O. , Hugelius Gustaf , Greger Maria , Smittenberg Rienk H. , Lyon Steve W. TITLE=Mojito, Anyone? An Exploration of Low-Tech Plant Water Extraction Methods for Isotopic Analysis Using Locally-Sourced Materials JOURNAL=Frontiers in Earth Science VOLUME=7 YEAR=2019 URL=https://www.frontiersin.org/journals/earth-science/articles/10.3389/feart.2019.00150 DOI=10.3389/feart.2019.00150 ISSN=2296-6463 ABSTRACT=

The stable isotope composition of water (δ18O and δ2H) is an increasingly utilized tool to distinguish between different pools of water along the soil-plant-atmosphere continuum (SPAC) and thus provides information on how plants use water. Clear bottlenecks for the ubiquitous application of isotopic analysis across the SPAC are the relatively high-energy and specialized materials required to extract water from plant materials. Could simple and cost-effective do-it-yourself “MacGyver” methods be sufficient for extracting plant water for isotopic analysis? This study develops a suite of novel techniques for plant water extraction and compares them to a standard research-grade water extraction method. Our results show that low-tech methods using locally-sourced materials can indeed extract plant water consistently and comparably to what is done with other state-of-the-art methods. Further, our findings show that other factors play a larger role than water extraction methods in achieving the desired accuracy and precision of stable isotope composition: (1) appropriate transport, (2) fast sample processing and (3) efficient workflows. These results are methodologically promising for the rapid expansion of isotopic investigations, especially for citizen science and/or school projects or in remote areas, where improved SPAC understanding could help manage water resources to fulfill agricultural and other competing water needs.