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ORIGINAL RESEARCH article

Front. Drug Saf. Regul.
Sec. Maternal-Fetal Medicine
Volume 4 - 2024 | doi: 10.3389/fdsfr.2024.1480515
This article is part of the Research Topic Enhancing Drug Safety for Pregnant and Lactating Women: Addressing Perinatal Pharmacotherapy Challenges View all articles

Detection of Nontoxic BoNT/A Levels in Post-Facial Botox Injection Breastmilk

Provisionally accepted
Helene Gu Helene Gu 1Zhenyu Xu Zhenyu Xu 1Renata Koviazina Renata Koviazina 1Pengcheng Tan Pengcheng Tan 1Changcheng Zheng Changcheng Zheng 1Ferdinand Kappes Ferdinand Kappes 1Domna G Kotsifaki Domna G Kotsifaki 1Fangrong Shen Fangrong Shen 2Anastasia Tsigkou Anastasia Tsigkou 1*
  • 1 Duke Kunshan University, Kunshan, China
  • 2 The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    The use of cosmetic Botox (BoNT/A) has become increasingly prevalent among women, even during the post-pregnancy breastfeeding period. However, there is currently a limited understanding of the extent Botox enters breastmilk and its potential effect on the breastfeeding infant. In this study, breastmilk samples were acquired from five women aged between 28-45. Three sample sets ranged from 1 hour to 1 year after facial Botox treatments (64 U), whereas the remaining two were from women who never received Botox. BoNT/A concentrations in samples were detected using standard Enzyme-Linked Immunosorbent Assay (ELISA), unreduced and reduced Western Blotting, confocal micro-Raman Spectroscopy, and Mass Spectrometry(LC-MS). From ELISA, the greatest breastmilk BoNT/A concentration was found from woman 1, 4 days after Botox injection (167 pg/mL). Levels were highest overall in the first week (82.45-167 pg/mL) and around two months (132.725 pg/mL) after injection. No clear indication of BoNT/A was detected in Mass Spectrometry (LC-MS), Western Blotting and

    Keywords: breastfeeding, Botulinum neurotoxin type A, ELISA, western blot, Confocal micro-Raman spectroscopy, Mass spectrometry (LC-MS)

    Received: 14 Aug 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 Gu, Xu, Koviazina, Tan, Zheng, Kappes, Kotsifaki, Shen and Tsigkou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Anastasia Tsigkou, Duke Kunshan University, Kunshan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.