ORIGINAL RESEARCH article

Front. Dent. Med.

Sec. Oral-Systemic Immunology

Volume 6 - 2025 | doi: 10.3389/fdmed.2025.1590516

This article is part of the Research TopicGenNext: The Future of Salivary ResearchView all 3 articles

Multiparameter Flow Cytometry Enables Immune Profiling and IFN Pathway Analysis in Human Minor Salivary Glands

Provisionally accepted
Eiko  YamadaEiko YamadaKalie  DominickKalie DominickRachel  J KulcharRachel J KulcharZohreh  KhavandgarZohreh KhavandgarMargaret  BeachMargaret BeachEileen  PelayoEileen PelayoAlan  N. BaerAlan N. BaerPaola  PerezPaola PerezBlake  Matthew WarnerBlake Matthew Warner*
  • National Institutes of Health (NIH), Bethesda, United States

The final, formatted version of the article will be published soon.

Methods articles present either a new or established method, protocol, or technique that is of significant interest in the field. Methods articles are peer-reviewed, have a maximum word count of 12,000 and may contain no more than 15 Figures/Tables. Authors are required to pay a fee (Atype article) to publish a Methods article. Method articles should have the following format: 1) Abstract, 2) Introduction (outlining the protocol and its possible applications), 3) Materials and Equipment (including a list of reagents/ materials and/or equipment required; formulation of any solutions where applicable), 4) Methods (including objectives and validation of the method; stepby-step procedures; timing of each step or related series of steps; pause points; example(s) of application and effectiveness; details of precision/ accuracy and limits of detection or quantification, where applicable) , 5) (Anticipated) Results (describing and illustrating with figures, where possible, the expected outcome of the protocol; advantages, limitations, possible pitfalls and artifacts and any troubleshooting measures to counteract them), 6) Discussion. Any analytical methods applied to the data generated by the protocol must be referenced or described. Results must be replicable.

Keywords: Salivary Glands, Sjogren's disease, Tissue dissociation, IFN Pathway Cytometry, Flow cyotmetry

Received: 09 Mar 2025; Accepted: 15 Apr 2025.

Copyright: © 2025 Yamada, Dominick, Kulchar, Khavandgar, Beach, Pelayo, Baer, Perez and Warner. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Blake Matthew Warner, National Institutes of Health (NIH), Bethesda, United States

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