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ORIGINAL RESEARCH article

Front. Dent. Med.

Sec. Periodontics

Volume 6 - 2025 | doi: 10.3389/fdmed.2025.1535753

This article is part of the Research Topic Diagnostic and Treatment Strategies for Periodontal Disease View all 5 articles

Antibiofilm Potential of Plant Extracts: Inhibiting Oral Microorganisms and Streptococcus mutans

Provisionally accepted
  • 1 Department of Prosthodontics, Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany
  • 2 Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
  • 3 Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • 4 Center of Dental Medicine, Faculty of Medicine, University of Zurich, Zürich, Zürich, Switzerland

The final, formatted version of the article will be published soon.

    A range of disinfectant mouthwashes are available for oral hygiene. The gold standard is Chlorhexidine digluconate (CHX), which, like other available products, cannot be used without side effects in the long term. However, in recent years, therapy with herbal products, often considered antiquated, has regained considerable interest. Therefore, the search for plant compounds as an alternative to existing oral disinfectants is meaningful. In this study, eleven Mediterranean plant extracts were tested for their antimicrobial effect in vitro. Methanol extracts of the following plants were produced by the pharmaceutical faculty of the University of Athens: Mentha aquatica, Mentha longifolia, Sideritis euboea, Sideritis syriaca, Stachys spinosa, Satureja parnassica, Satureja thymbra, Lavandula stoechas, Achillea taygetea, Phlomis cretica, and Vaccinium myrtillus. The extracts were dissolved for microdilution experiments at concentrations ranging from 10 mg/ml to 0.019 mg/ml. The oral pathogens tested were Streptococcus mutans, Streptococcus oralis, Streptococcus sobrinus, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas gingivalis, and Candida albicans. Enterococcus faecalis, Staphylococcus aureus, and Escherichia coli were used as references.All extracts, except the methanol extract of V. myrtillus, showed an antibacterial effect at concentrations ranging from 10 mg/ml to 0.15 mg/ml. None of the extracts exhibited a significant antifungal effect. In general, the anaerobic pathogens could be inhibited and killed at lower concentrations compared to the aerobic pathogens. S. oralis also showed good susceptibility to the extracts. Additionally, the extracts' ability to inhibit biofilm formation by S. mutans was tested. L. stoechas at a concentration of 0.3 mg/ml showed a moderate inhibitory effect. The extracts of L. stoechas, S. thymbra, S. parnassica, and the methanol extract of V. myrtillus were effective at concentrations up to 1.25 mg/ml. P. cretica was able to inhibit and kill S. mutans at a concentration of 0.6 mg/ml, but its effectiveness in biofilm inhibition significantly decreased at 2.5 mg/ml. The study's hypothesis that all extracts would exhibit an antimicrobial effect was thus confirmed.

    Keywords: Mediterranean herb extracts, oral mouthwashes, Antimicrobial activity, biofilm inhibition, Streptococcus mutans

    Received: 27 Nov 2024; Accepted: 24 Mar 2025.

    Copyright: © 2025 Bartels, Argyropoulou, Al-Ahmad, Hellwig, Skaltsounis, Wittmer, Vach and Karygianni. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Lamprini Karygianni, Center of Dental Medicine, Faculty of Medicine, University of Zurich, Zürich, 8032, Zürich, Switzerland

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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