AUTHOR=Reis Caio Luiz Bitencourt , Barbosa Mariane Carolina Faria , de Lima Daniela Coelho , Madalena Isabela Ribeiro , Baratto-Filho Flares , Proff Peter , de Oliveira Daniela Silva Barroso , Paddenberg Eva , Küchler Erika Calvano , Kirschneck Christian 

TITLE=Study of Dental Caries and PTH Gene

JOURNAL=Frontiers in Dental Medicine

VOLUME=2

YEAR=2021

URL=https://www.frontiersin.org/journals/dental-medicine/articles/10.3389/fdmed.2021.703549

DOI=10.3389/fdmed.2021.703549

ISSN=2673-4915

ABSTRACT=<p>Parathyroid hormone (PTH) is essential for calcium and phosphate homeostasis in odontogenesis-related cells. Therefore, the present study aimed to investigate the association between single nucleotide polymorphisms in the gene encoding PTH, and dental caries in Brazilian children. Three hundred and fifty-three children (170 boys and 183 girls, age ranging from 8 to 11 years old) were included in this study. The International System for Detection and Assessment of Carious Lesions (ICDAS) was used for diagnosis of dental caries. Visible biofilm was also evaluated during the clinical examination. Genomic DNA was extracted from saliva for real-time PCR to evaluate the single nucleotide polymorphisms rs6256, rs307247 and rs694 in <italic>PTH</italic> gene. Dental caries was classified in ICDAS<sub>0</sub> vs. ICDAS<sub>1−6</sub> or ICDAS<sub>1−2</sub> vs. ICDAS<sub>3−6</sub>. Chi-square test, binary logistic regression adjusted by biofilm and haplotype analyses were performed (<italic>p</italic> &lt; 0.05). Biofilm was associated with dental caries (p &lt; 0.05). There were no associations between dental caries and rs6256, rs307247, rs694 in none of the analyses performed (<italic>p</italic> &gt; 0.05). In conclusion, the present study supports that the single nucleotide polymorphisms rs6256, rs307247, and rs694 in the PTH-encoding gene are not associated with dental caries in Brazilian children.</p>