AUTHOR=Schoeters Ruben , Tarnaud Thomas , Martens Luc , Joseph Wout , Raedt Robrecht , Tanghe Emmeric TITLE=Double Two-State Opsin Model With Autonomous Parameter Inference JOURNAL=Frontiers in Computational Neuroscience VOLUME=15 YEAR=2021 URL=https://www.frontiersin.org/journals/computational-neuroscience/articles/10.3389/fncom.2021.688331 DOI=10.3389/fncom.2021.688331 ISSN=1662-5188 ABSTRACT=
Optogenetics has a lot of potential to become an effective neuromodulative therapy for clinical applications. Selecting the correct opsin is crucial to have an optimal optogenetic tool. With computational modeling, the neuronal response to the current dynamics of an opsin can be extensively and systematically tested. Unlike electrical stimulation where the effect is directly defined by the applied field, the stimulation in optogenetics is indirect, depending on the selected opsin's non-linear kinetics. With the continuous expansion of opsin possibilities, computational studies are difficult due to the need for an accurate model of the selected opsin first. To this end, we propose a double two-state opsin model as alternative to the conventional three and four state Markov models used for opsin modeling. Furthermore, we provide a fitting procedure, which allows for autonomous model fitting starting from a vast parameter space. With this procedure, we successfully fitted two distinctive opsins (ChR2(H134R) and MerMAID). Both models are able to represent the experimental data with great accuracy and were obtained within an acceptable time frame. This is due to the absence of differential equations in the fitting procedure, with an enormous reduction in computational cost as result. The performance of the proposed model with a fit to ChR2(H134R) was tested, by comparing the neural response in a regular spiking neuron to the response obtained with the non-instantaneous, four state Markov model (4SB), derived by Williams et al. (