Baraa Mohammed Yaseen ¹ Farag M. A. Altalbawy ^2,3 Rafid Jihad Albadr ⁴ Waam Mohammed Taher ⁵ Mariem Alwan ⁶ Mahmood Jasem Jawad ⁷ Hiba Mushtaq ⁸ KHURSHEED MUZAMMIL ⁹ Ahmed Hussein Zwamel ^10,11,12*

• ¹ Department of Medical Laboratory Technics, AlNoor University College, Mosul, Nineveh, Iraq
• ² Department of Chemistry, College of Science, University College of Duba, University of Tabuk, Tabuk, Saudi Arabia
• ³ National Institute of Laser Enhanced Sciences, Cairo University, Cairo, Beni Suef, Egypt
• ⁴ Ahl al Bayt University , Kerbala , Iraq, Kerbala, Iraq
• ⁵ National University of Science and Technology, Thi-Qar, Iraq
• ⁶ Pharmacy college, Al-Farahidi University, Iraq, Baghdad, Iraq
• ⁷ Department of Pharmacy, Al-Zahrawi University College, Karbala, Iraq, Karbala, Iraq
• ⁸ Gilgamesh Ahliya University, Baghdad, Baghdad, Iraq
• ⁹ Department of Public Health, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia
• ¹⁰ Medical laboratory technique college, Islamic University of Najaf, Najaf, Iraq
• ¹¹ Medical Laboratory Technique College, Islamic University of Al Diwaniyah, Al Diwaniyah, Al-Qādisiyyah, Iraq
• ¹² Medical laboratory technique college, the Islamic University of Babylon, Babylon, Iraq, Babylon, Iraq

In this study, a new MOF (metal-organic framework) based on vanadium and 2,2-bipyridine-4,4dicarboxylic acid (V/BP-MOF) was synthesized. Synthesized V/BP-MOF was introduced as a strong adsorbent of Congo Red (CR) and an effective agent in eliminating microbial species. In the investigation of CR absorption activity, several factors such as concentration of V/BP-MOF, pH, time, and temperature were investigated. Antimicrobial evaluations were carried out on Common bacterial strains in wastewater and values of MIC (minimum inhibitory concentration) and MBC (Minimum Bactericidal Concentration) were reported. The V/BP-MOF was confirmed and characterized by EA, EDS, EDS mapping, FT-IR, XRD, TGA, BET, SEM, and TEM. In checking the characteristics of V/BP-MOF, size, specific surface area, and thermal stability were obtained, respectively, 68 nm, 325 m 2 /g, and 320 °C. The highest adsorption of CR, at 94%, was obtained at natural pH, ambient temperature, and after 150 min. In kinetic studies, a correlation coefficient of 0.99 was observed with the pseudo-second-order kinetic model, while in isotherm studies, a correlation coefficient of 0.97 was observed with the Freundlich isotherm model. In the biological evaluations, the best inhibition was against Escherichia coli, and MIC and MBC were observed as 4 μg/mL and 2 μg/mL, respectively. As a general result, V/BP-MOF can be introduced as a potent absorbent agent of CR dye and antimicrobial properties. Therefore, the compound synthesized in this study can be introduced as a suitable option for the wastewater treatment industry, with multiple capabilities including the removal of chemical pollutants and pathogenic agents.