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ORIGINAL RESEARCH article
Front. Chem.
Sec. Analytical Chemistry
Volume 13 - 2025 |
doi: 10.3389/fchem.2025.1523712
This article is part of the Research Topic Advances in Secondary Ion Mass Spectrometry – Volume II View all articles
A Laboratory-Friendly Protocol for Freeze-Drying Sample Preparation in ToF-SIMS Single-Cell Imaging
Provisionally accepted- Minzu University of China, Beijing, China
ToF-SIMS is a high spatial resolution imaging technique for cellular or subcellular analysis of biological samples. Accurate molecular data in single-cell studies depend on proper cell morphology and chemical integrity, highlighting the importance of sample preparation. In this work, we standardized a more efficient freeze-drying method using standard lab materials and improved the sample preparation process. Our comprehensive freeze-drying protocol for cellular samples, encompassing washing, fixation, and drying steps, facilitates the acquisition of enhanced cellular information and ensures high reproducibility. These improvements are poised to significantly advance single cell mass spectrometry imaging research.
Keywords: ToF-SIMS, Single cell imaging, sample preparation, Freeze-drying, Mass spectrometry imaging
Received: 06 Nov 2024; Accepted: 23 Jan 2025.
Copyright: © 2025 Shi, Liu, Qi, Ma, Wang, Chen and Abliz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhaoying Wang, Minzu University of China, Beijing, China
Yanhua Chen, Minzu University of China, Beijing, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.