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ORIGINAL RESEARCH article
Front. Chem.
Sec. Medicinal and Pharmaceutical Chemistry
Volume 13 - 2025 | doi: 10.3389/fchem.2025.1425666
This article is part of the Research Topic Antibacterial Effects and Mode of Action of New Active Substances Against Drug Resistant Pathogenic Bacteria View all 5 articles
Exploring the antimicrobial potential of lactobacilli against early-stage and mature biofilms of Staphylococcus aureus and Pseudomonas aeruginosa
Provisionally accepted
NIHARIKA SINGH 1
Rohini Devidas Gulhane 1
Maitri Goel 1
Pudke Payal Udelal 1 Anamika Singh 1 Vikas Sangwan 1
Manvesh Kumar Sihag 1
Gunjan Goel 2
Harsh Panwar 1
Anil Kumar Puniya 3*- 1 Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
- 2 Central University of Haryana, Haryana, Haryana, India
- 3 National Dairy Research Institute (ICAR), Karnal, India
Bacterial biofilms are dynamic, complex and very adaptive, and can cause harm to health problems in both human and animals; besides posing a serious threat to industry. This study presents the potential of cell free preparations of lactobacilli isolated from breast milk (HM; n=11) and infant faecal (IF; n=15) samples to impact the growth of Staphylococcus aureus and Pseudomonas aeruginosa biofilms. The anti-biofilm activity of three distinct cell free preparations viz. un-treated cell free supernatant (CFS), pH neutralized CFS (N-CFS), and heat-treated CFS (H-CFS) was examined against both early stage and mature biofilms. While the post-incubation strategy examined the impact on mature biofilms, the co-incubation treatment assessed the impact of CFS on adhesion and initial colonization. When compared to post-incubation treatment (HM3; 67.12 %), the CFSs exhibited greater inhibitory activity during co-incubation (IF9; 85.19%). The un-treated CFS exhibited most promising biofilm inactivation; while the activity was not completely lost upon pH neutralization and heat treatment. After treating with H-CFSs and N-CFSs, the population of S. aureus and P. aeruginosa bacterial cells in the biofilm moderately decreased by 40-60%. Microscopic observations showed that after CFS challenge, the integrity of the biofilm conformation was destroyed. According to principal component analysis (significance level at p < 0.05), the most promising anti-biofilm activity against both test pathogens was found in the CFS of Lacticaseibacillus paracasei HM1.
Keywords: Lactic acid bacteria, antimicrobial, probiotics, Cell free supernatant, Biofilm, Food-borne pathogens, MTT assay, Staphylococcus aureus, Pseudomonas aeruginosa
Received: 30 Apr 2024; Accepted: 31 Jan 2025.
Copyright: © 2025 SINGH, Gulhane, Goel, Udelal, Singh, Sangwan, Sihag, Goel, Panwar and Puniya. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Anil Kumar Puniya, National Dairy Research Institute (ICAR), Karnal, India
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