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ORIGINAL RESEARCH article

Front. Chem.
Sec. Analytical Chemistry
Volume 12 - 2024 | doi: 10.3389/fchem.2024.1490639
This article is part of the Research Topic Recent Advances in Pharmaceutical Analysis: Applications and New Challenges for the Quality of Medicines View all 5 articles

INNOVATIVE DETERMINATION OF PHYTOHORMONES IN ALOE VERA

Provisionally accepted
ILTAF SHAH ILTAF SHAH *Muhammad K Hakeem Muhammad K Hakeem Meera Maraqa Meera Maraqa Sampath K Elangovan Sampath K Elangovan
  • United Arab Emirates University, Al-Ain, United Arab Emirates

The final, formatted version of the article will be published soon.

    This article introduces a novel approach on the determination of six phytohormones (Abscisic Acid (ABA), Salicylic Acid (SA), Indole-3-Acetic Acid (I3AA), Gibberellic Acid (GA), 6-Benzylaminopurine (6BAP), And Isopentenyladenine (ISA)) in Aloe vera using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Aloe vera is widely recognized for its therapeutic properties, but concerns regarding phytohormone levels and their potential impact on human health necessitate advanced analytical techniques. This study presents a validated LC-MS/MS method designed specifically for the determination and quantification of phytohormones in Aloe vera. The optimization of sample extraction and mobile phase composition resulted in enhanced chromatographic separation and improved mass spectrometry (MS) sensitivity. Utilizing a C-18 column for separation and a triple quadrupole mass spectrometer for determination and quantitation, the method demonstrated excellent linearity (R² > 0.99) and achieved low limits of detection for all six analytes (ABA, SA, I3AA, GA, 2NAA, 6BAP). Results revealed that four of the six analyzed phytohormones consistently emerged as predominant. Quantitative analysis showed that ABA exhibited the highest median concentration (8.39 ng/mL), followed by I3AA (4.32 ng/mL), SA (3.16 ng/mL), and GA (1.55 ng/mL), highlighting their predominant roles in Aloe vera's phytohormonal profile. By presenting this advanced LC-MS/MS methodology, our study not only addresses concerns related to the levels of phytohormones in Aloe vera but also contributes a valuable tool for the comprehensive characterization of phytohormonal profiles in diverse plant species.

    Keywords: LC-MS/MS, aloe vera, phytohormones, liquid chromatography mass spectrometry, Plant hormone, Method validation

    Received: 05 Sep 2024; Accepted: 12 Dec 2024.

    Copyright: © 2024 SHAH, Hakeem, Maraqa and Elangovan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: ILTAF SHAH, United Arab Emirates University, Al-Ain, United Arab Emirates

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.