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ORIGINAL RESEARCH article

Front. Chem.
Sec. Green and Sustainable Chemistry
Volume 12 - 2024 | doi: 10.3389/fchem.2024.1469271
This article is part of the Research Topic Sustainable Synthesis for Obtaining Elements of Natural Origin with Antimicrobial Properties View all articles

Synthesis of chitosan nanoparticles (CSNP): Effect of CH-CH-TPP ratio on size and stability of NPs

Provisionally accepted
Rosvin Des Bouillons-Gamboa Rosvin Des Bouillons-Gamboa 1Gabriela Montes De Oca-Vásquez Gabriela Montes De Oca-Vásquez 2JOSE ROBERTO V. BAUDRIT JOSE ROBERTO V. BAUDRIT 2*Liz Carolina Ríos Duarte Liz Carolina Ríos Duarte 3Mary Lopretti Mary Lopretti 4Maite Renteria Urquiza Maite Renteria Urquiza 5Juan Miguel Zúñiga-Umaña Juan Miguel Zúñiga-Umaña 2Maria-Filomena Barreiro Maria-Filomena Barreiro 6Patricia Vazquez Patricia Vazquez 10,7,8,9
  • 1 School of Biology, Tecnológico de Costa Rica, Cartago, Costa Rica
  • 2 LANOTEC-CENAT, San Jose, Costa Rica
  • 3 National University of Asunción, Research Deptment of Food Engineering and Technology, San Lorenzo, Paraguay
  • 4 Center for Nuclear Research, , University of the Republica, Montevideo, Uruguay
  • 5 Department of Chemistry, University of Guadalajara, Guadalajara, Mexico
  • 6 Polytechnic Institute of Bragança (IPB), Bragança, Portugal
  • 7 Universidad de La Plata, Argentina, Argentina
  • 8 Universidad de La Plata, La Plata, Argentina
  • 9 Universidad Nacional de la Plata, La Plata, Argentina
  • 10 UNLP, La Plata, Argentina

The final, formatted version of the article will be published soon.

    In the face of a pressing global issue-the escalating threat of antibiotic resistance-the development of new antimicrobial agents is urgent. Nanotechnology, with its innovative approach, emerges as a promising solution to enhance the efficacy of these agents and combat the challenge of microbial resistance. Chitosan nanoparticles (CSNPs) stand out in biomedical applications, particularly in the controlled release of antibiotics, with their unique properties such as biocompatibility, stability, biodegradability, non-toxicity, and simple synthesis processes suitable for sensitive molecules. This study synthesized CSNPs using the ionotropic gelation method, with tripolyphosphate (TPP) as the crosslinking agent. Various CS: TPP ratios (6:1, 5:1, 4:1, 3:1, 2:1) were tested, and the resulting nanoparticles were evaluated using dynamic light scattering (DLS). The CS: TPP ratio of 4:1, with an average hydrodynamic diameter (DHP) of (195±10) nm and a zeta potential of (51±1) mV, was identified as the most suitable for further analysis. The characterization of NPs by transmission electron microscopy (TEM) and atomic force microscopy (AFM) revealed diameters of (65±14) nm and (102±18) nm, respectively. Notably, CSNPs exhibited significant aggregation during

    Keywords: Chitosan, Nanoparticles, Synthesis, antibiotic resistance, AFM, DLS, TEM

    Received: 23 Jul 2024; Accepted: 18 Oct 2024.

    Copyright: © 2024 Des Bouillons-Gamboa, Montes De Oca-Vásquez, BAUDRIT, Ríos Duarte, Lopretti, Renteria Urquiza, Zúñiga-Umaña, Barreiro and Vazquez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: JOSE ROBERTO V. BAUDRIT, LANOTEC-CENAT, San Jose, Costa Rica

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