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ORIGINAL RESEARCH article

Front. Chem.
Sec. Medicinal and Pharmaceutical Chemistry
Volume 12 - 2024 | doi: 10.3389/fchem.2024.1462309

UPLC-ESI/MS n Metabolic Profiling of Cedrela odorata L. and Toona ciliata M. Roem and in vitro Investigation of their Anti-diabetic Activity Supported with Molecular Docking Studies

Provisionally accepted
Heba A. El-Nashar Heba A. El-Nashar 1*Esraa A. Elhawary Esraa A. Elhawary 1Ayman M. Al-Qaaneh Ayman M. Al-Qaaneh 2Md. Shimul Bhuia Md. Shimul Bhuia 3Raihan Chowdhury Raihan Chowdhury 3Mostafa Abdel-Maksoud Mostafa Abdel-Maksoud 4Abdul Malik Abdul Malik 4Muhammad Torequl Islam Muhammad Torequl Islam 3Mohammeed Aufy Mohammeed Aufy 5
  • 1 Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
  • 2 Al-Balqa Applied University, Al-Salt, Balqa, Jordan
  • 3 Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, Bangladesh
  • 4 King Saud University, Riyadh, Riyadh, Saudi Arabia
  • 5 University of Vienna, Vienna, Vienna, Austria

The final, formatted version of the article will be published soon.

    Genus Cedrela is one of the phytochemically rich genera of Family Meliaceae. In this study, two Cedrela species, namely, Cedrela odorata and Toona ciliata M. Roem (formerly Cedrela toona) were selected for in-depth phytochemical profiling with the aid of UPLC-ESI/MS analysis followed by evaluation of their anti-diabetic potential through assessment of in vitro α-amylase and α-glucosidase inhibitory effects, alongside the molecular docking studies on these target enzymes. The UPLC-ESI/MS n analysis led to the identification and quantification of fifty-five metabolites with their fragmentation patterns for the first time for these two species. Flavonoids represented the main identified class followed by phenylpropanoids, terpenes, tannins, and others.The two species showed potent enzyme inhibition where C. odorata and C. toona significantly inhibited α-amylase (IC50=4.83±0.01 and 3.50±0.03 µg/mL), respectively, compared to pioglitazone (IC50=2.17±0.23 µg/mL) while their α-glycosidase inhibitory properties were also potent with (IC50=7.17±0.01 and 6.50±0.69 μg/mL), respectively, compared to acarbose (IC50=4.83±1.02 μg/mL). The enzyme inhibitory activities were further confirmed by in silico molecular docking of the main identified components with the respective binding sockets in both α-amylase and α-glycosidase enzymes. These promising results could pave the way for a new discovery of natural phytoconstituents with potent anti-diabetic activity.

    Keywords: Cedrela, UPLC-ESI/MS n, anti-diabetic, α-amylase, α-glucosidase, phenylpropanoids

    Received: 09 Jul 2024; Accepted: 10 Oct 2024.

    Copyright: © 2024 El-Nashar, Elhawary, Al-Qaaneh, Bhuia, Chowdhury, Abdel-Maksoud, Malik, Islam and Aufy. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Heba A. El-Nashar, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.