AUTHOR=Murata Takatsugu , Komukai Kyoka , Semba Yuta , Murata Eri , Sato Fumi , Takano Tomohiro , Tsuchiya Kaho , Matsuda Chihiro , Sakai Anju , Yoneoka Amane , Takahashi Shunsuke , Nagahara Yukitoshi , Shiina Isamu 

TITLE=Synthesis of BODIPY FL-tethered ridaifen-B, RID-B-BODIPY, and its localization in cancer cells

JOURNAL=Frontiers in Chemistry

VOLUME=12

YEAR=2024

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2024.1451468

DOI=10.3389/fchem.2024.1451468

ISSN=2296-2646

ABSTRACT=<p>We synthesized ridaifen-B boron dipyrromethene (<bold>RID-B-BODIPY</bold>) using 2-methyl-6-nitro benzoic anhydride (MNBA)-mediated dehydration condensation reaction between amino alkyl-tethered RID and BODIPY FL. Comparative experiments between dicyclohexylcarbodiimide (DCC) and MNBA for their coupling reactions demonstrated that MNBA is an effective condensation reagent for amines and BODIPY FL. A cell staining study with <bold>RID-B-BODIPY</bold> showed intracellular localization of BODIPY FL fluorescence, attributed to the <bold>RID-B</bold> structure, indicating the successful development of a tool for analyzing intracellular molecular behavior efficiently.</p>