Syed Shams ul Hassan ^1* Xiao Zhang ¹ Jiajia Wu ¹ Abdur Rehman ²

• ¹ Shanghai Jiao Tong University, Shanghai, China
• ² Northwest A&F University, Xianyang, Shaanxi Province, China

Elicitation through abiotic stress, including heavy metals, is a new technique for drug discovery. In this research, one potent antibiotic was achievedThis research achieved one potent antibiotic through triggering ofby triggering nickel chloride on in marine bacteria. The compound CDPDP CDPDP has shown potent antibacterial activity against Staphylococuus aureus and Riemerella anatipestifer with a bactericidal effect on 300 µm. The compound has good bioactivity score prediction and drug drug-likeness.To know investigate the anti-bacterial mechanism, , molecular docking was performedthe compound has been docked with four proteins, including PBP2a , bacterial DNA gyrase, tRNA N6-adenosine threonylcarbamoyltransferase, and RMFPRMFP, and the highest docking score was achieved against RMFP with a score of -12.2 kcal/mol. Furthermore, protein-protein docking was performed with and TLR-4 protein for predicting vaccine construction, and the best docking score was achieved against PBP2a and RMFP enzyme with highest score of -12.2 against RMFP. In-silico cloning was carried out to Formatted: Romanian ensure the expression of proteins generated and were cloned using E. coli as a host. The simulation studies have shown that both CDPDP-RMFP and TLR-4-RMFP complexes are stable with the system. This is the first study showing anti-bacterial drug discovery from bacteria through heavy metals triggering and mechanism studies of Riemerella anatipestifer.