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ORIGINAL RESEARCH article

Front. Chem.
Sec. Chemical Biology
Volume 12 - 2024 | doi: 10.3389/fchem.2024.1417407
This article is part of the Research Topic Natural Products from Plants or Microorganisms for the Treatment of Non-Communicable Diseases View all 3 articles

Development of Polyvinyl Alcohol Nanofiber Scaffolds Loaded with Flaxseed Extract for Bone Regeneration: Phytochemicals, Cell Proliferation, Adhesion, and Osteogenic Gene Expression

Provisionally accepted
Ahmed G. Abdelaziz Ahmed G. Abdelaziz 1Hassan Nageh Hassan Nageh 2Mohga S. Abdalla Mohga S. Abdalla 1Sara M. Abdo Sara M. Abdo 1Asmaa Amer Asmaa Amer 3Samah A. Loutfy Samah A. Loutfy 2Nasra Abdel Fattah Nasra Abdel Fattah 4Ali Alsalme Ali Alsalme 5David CORNU David CORNU 6Mikhael Bechelany Mikhael Bechelany 6Ahmed Barhoum Ahmed Barhoum 1*
  • 1 Helwan University, Helwan, Cairo, Egypt
  • 2 British University in Egypt, Cairo, Cairo, Egypt
  • 3 National Research Centre (Egypt), Cairo, Cairo, Egypt
  • 4 National Cancer Institute, Cairo, Egypt
  • 5 King Saud University, Riyadh, Riyadh, Saudi Arabia
  • 6 UMR5635 Institut Européen des Membranes (IEM), Montpellier, Languedoc-Roussillon, France

The final, formatted version of the article will be published soon.

    This study assessed the incorporation of a flaxseed extract into electrospun polyvinyl alcohol (PVA) nanofibers to produce nanofiber scaffolds for bone tissue engineering. High-performance liquid chromatography analysis of the total flaxseed extract revealed the presence of ten compounds, including polyphenolic acids and flavonoids. The diameter of the resulting nanofibrous structures ranged from 252 nm for pure PVA nanofibers to 435 nm for PVA nanofibers loaded with 30 wt.% flaxseed extract (P70/E30). Their function as scaffolds was evaluated in MG-63 osteoblast cultures. The optimization of key nanofiber characteristics (diameter, extract concentration, hydrophilicity, swelling behaviour, and hydrolytic degradation) enhanced the scaffold's efficacy in supporting cell adhesion, proliferation, and differentiation.Flaxseed extract-loaded nanofibers promoted cell proliferation/viability (MTT assay) compared with pure PVA nanofibers: cell viability was 74.5% for PVA nanofibers and 102.6% for P70/E30 nanofibers (1 mg/mL for both). However, flaxseed extract incorporation reduced cell adhesion (206 cells/section for PVA nanofibers vs 151 for P70/E30 nanofibers) due to the scaffold hydrophilicity decrease after extract loading. Importantly, the nanofibrous scaffolds induced MG-63 osteoblast differentiation, as indicated by the significant upregulation of osteogenic genes (RT-qPCR analysis): 1.2-fold and 106.8-fold for RUNX2, 1.6-fold and 25.9-fold for COL1A1, and 2.4fold and 16-fold for OCN with PVA and P70/E30 nanofibers, respectively, compared to control without scaffolds. The tailored optimization of the nanofibrous scaffold properties holds promise for regenerative medicine applications.

    Keywords: Flaxseed extract, bone tissue engineering, Electrospinning, Nanofibrous scaffold, Cell Proliferation, osteoblast differentiation, Regenerative Medicine, MTT

    Received: 14 Apr 2024; Accepted: 04 Jul 2024.

    Copyright: © 2024 Abdelaziz, Nageh, Abdalla, Abdo, Amer, Loutfy, Abdel Fattah, Alsalme, CORNU, Bechelany and Barhoum. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ahmed Barhoum, Helwan University, Helwan, 11792, Cairo, Egypt

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.