Gongzhen Liu ^1* Kangyu Zhong ¹ Shanmin Gong ² Xinru Li ² Yanshen Li ^2*

• ¹ Linyi University, Linyi, China
• ² Yantai University, Yantai, Shandong Province, China

Cipangopaludina chinensis is a common freshwater mollusk that is widely distributed worldwide, especially in China. In our research, a total of 1,382 proteins and 1,039 phosphorylated proteins were identified from C. chinensis carcasses, and 690 differentially expressed proteins (DEPs) were quantified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that the DEPs are involved in cellular processes, single-organism processes, metabolic processes, developmental processes, localization, and biological regulation. The phosphorylated proteins were found to be related to the Rap1 signaling pathway, Ras signaling pathway, calcium signaling pathway, and longevity regulating pathways, among others. Moreover, we also identified important regulatory enzymes, such as Guanylate cyclase, Tyrosine-protein kinase, Receptor protein-tyrosine kinase, and Glyoxylate reductase/Hydroxypyruvate reductase. Notably, we found Guanylate cyclase to be present in multiple signaling pathways, including the Rap1 signaling pathway, Calcium signaling pathway, Ras signaling pathway, Insulin secretion, Longevity regulating pathway, Glutamatergic synapse, Circadian entrainment, and Gap junction. This enzyme may play a crucial role in regulating molecular mechanisms in C. chinensis. In summary, the proteomics and phosphoproteomics analysis of C. chinensis carcasses displayed significant differences among different geographical isolates, which helps to enhance our understanding of food nutrition, signaling pathways, and metabolic mechanisms in C. chinensis.