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ORIGINAL RESEARCH article

Front. Chem.
Sec. Analytical Chemistry
Volume 12 - 2024 | doi: 10.3389/fchem.2024.1409420
This article is part of the Research Topic Mass Spectrometry Imaging Towards Multimodal Imaging View all 5 articles

A novel rhodamine B fluorescence probe for rapid identification of different amino acids by high efficiency fluorescence spectrum-mass spectrometry

Provisionally accepted
Xiujie Duan Xiujie Duan 1Tao Jin Tao Jin 2Boneng ao Boneng ao 2Shihe Shao Shihe Shao 2Zhao Lei Zhao Lei 3*
  • 1 Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
  • 2 Yixing People's Hospital, Yixing, Jiangsu, China
  • 3 School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, China

The final, formatted version of the article will be published soon.

    Rapid detection of amino acids plays an important role in the field of medical diagnosis. By combining Rhodamine B with triphenylamine, a novel double-response fluorescence probe (E)-4- ((4-(((3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)imino)methyl)phenyl)(phe nyl)amino)benzaldehyde (RBTPA) was prepared for rapid identification of different amino acids.Under daylight and 365 nm irradiation, it was found that the color change was most bright at pH=3, and changed to dim at pH=4. When pH=3 and pH= 4, the photophysical properties of the two strong acids are very different. The maximum redshift of UV absorption light is 110 nm, and the maximum fluorescence emission intensity is 4 times different. In order to further observe their binding structure analysis with different amino acids, qualitative analysis of each response structure was determined by mass spectrometry according to different molecular weights. The fluorescence probe RBTPA has two different isomers for recognition response in aldehyde group and imine group, respectively.

    Keywords: Rhodamine B, Rapid identification, different amino acid, Fluorescence spectrum, mass spectrometry. GA

    Received: 30 Mar 2024; Accepted: 20 Sep 2024.

    Copyright: © 2024 Duan, Jin, ao, Shao and Lei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhao Lei, School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.