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ORIGINAL RESEARCH article

Front. Chem.
Sec. Nanoscience
Volume 12 - 2024 | doi: 10.3389/fchem.2024.1406869
This article is part of the Research Topic Bioactive Containing Plant Based Waste for (Nano)-Biocomposites: Applications in Biomedicine, Health, and Bioremediation View all 3 articles

Bioactive Plant waste Components Targeting Oral Bacterial Pathogens as a Promising Strategy for Biofilm Eradication

Provisionally accepted
Saima Mashal Saima Mashal 1Dr Aisha Siddiqua Dr Aisha Siddiqua 2Niamat Ullah Niamat Ullah 3Rabia Baloch Rabia Baloch 4Momin Khan Momin Khan 5Zia U. Syed Zia U. Syed 6Muhammad Imran Aziz Muhammad Imran Aziz 3Adnan Amin Adnan Amin 2*Elchin Huseynov Elchin Huseynov 7Dragica Selakovic Dragica Selakovic 8Gvozden Rosic Gvozden Rosic 8Trobjon Makhkamov Trobjon Makhkamov 9Akramjon Yuldashev Akramjon Yuldashev 10Sokhib Islamov Sokhib Islamov 9Nilufar Abdullayeva Nilufar Abdullayeva 11Uktam Khujanazarov Uktam Khujanazarov 9
  • 1 Gomal Center of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan
  • 2 Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan
  • 3 Faculty of Pharmacy, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan
  • 4 D.G Khan Medical College, Punjab, Punjab, Pakistan
  • 5 Khyber Medical University, Peshawar, Khyber Pakhtunkhwa, Pakistan
  • 6 Bahauddin Zakariya University, Multan, Punjab, Pakistan
  • 7 Azerbaijan Medical University, Baku, Azerbaijan
  • 8 University of Kragujevac, Kragujevac, Serbia
  • 9 Tashkent State Pedagogical University, Tashkent, Tashkent, Uzbekistan
  • 10 Andijan State University, Andijan, Uzbekistan
  • 11 Jizzakh State Pedagogical Institute, Jizzakh, Jizzakh, Uzbekistan

The final, formatted version of the article will be published soon.

    The significance of this study lies in its exploration of bioactive plant extracts as a promising avenue for combating oral bacterial pathogens, offering a novel strategy for biofilm eradication that could potentially revolutionize oral health treatments. Oral bacterial infections are common in diabetic patients, however due to development of resistance treatment options are limited. Keeping in mind excellent antimicrobial properties of phenolic compounds, we investigated them against isolated oral pathogens using in silico and in vitro models. We performed antibiogram studies, minimum inhibitory concentration (MIC), antibiofilm and antiquorum sensing activities covering phenolic compounds. Bacterial strains were isolated from female diabetic patients and identified by using16s rRNA as Pseudomonas aeruginosa, Bacillus chungangensis, Bacillus paramycoides, Bacillus chungangensis and Paenibacillus dendritiformis. Antibiograms studies confirmed that all strains were resistant to most of tested antibiotics except imipenem and ciprofloxacin. Molecular docking analysis revealed significant interaction of rutin, quercetin, gallic acid and catechin with transcription regulator genes 1RO5, 4B2O and 5OE3. All tested molecules followed drug likeness rules except rutin.MIC values of tested compounds varied from 0.0625 to 0.5 mg/mL against clinical isolates. A significant antibiofilm activity was recorded in case of catechin (73.5±1.6% inhibition against Bacillus paramycoides) cinnamic acid (80.9±1.1% inhibition against Pseudomonas aeruginosa), vanillic and acid quercetin (65.5±1.7% and 87.4±1.4% inhibition against Bacillus chungangensis) at 0.25-0.125 mg/mL. None of the phenolic compounds presented antiquorum sensing activity. It was therefore concluded that polyphenolic compounds may have potential to be used against oral bacterial biofilms and further detailed mechanistic investigations can be performed.

    Keywords: Oral pathogens, bioremediation, polyphenolic compounds, Docking, Paenibacillus dendritiformis

    Received: 25 Mar 2024; Accepted: 18 Jul 2024.

    Copyright: © 2024 Mashal, Siddiqua, Ullah, Baloch, Khan, Syed, Imran Aziz, Amin, Huseynov, Selakovic, Rosic, Makhkamov, Yuldashev, Islamov, Abdullayeva and Khujanazarov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Adnan Amin, Gomal University, Dera Ismail Khan, 29050, Khyber Pakhtunkhwa, Pakistan

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.