Skip to main content

ORIGINAL RESEARCH article

Front. Chem.
Sec. Analytical Chemistry
Volume 12 - 2024 | doi: 10.3389/fchem.2024.1386635

Establishment and evaluation of a UPLC-MS/MS method for simultaneous determination of bongkrekic acid and dehydroacetic acid in rice noodles

Provisionally accepted
Lingguo Zhao Lingguo Zhao 1Lv Ronggui Lv Ronggui 2*Jiang Song Jiang Song 2Chen Xi Chen Xi 2Lei lei Lei lei 3Zeng Weixian Zeng Weixian 2She Miaowen She Miaowen 4Li Dabin Li Dabin 5Yu Xiaxia Yu Xiaxia 6Liu Zhanguo Liu Zhanguo 7Wang Xiao Wang Xiao 8,9Liu Yong Liu Yong 2
  • 1 Ban'an District Center for Disease Control and Prevention, Shenzhen, China
  • 2 Shenzhen Hospital, Southern Medical University, Shenzhen, China
  • 3 Shenzhen Bao'an Disease Prevention and Control Center, Shenzhen, Guangdong Province, China
  • 4 Taihe People's Hospital, Fuyang, Anhui, China
  • 5 Kiang Wu Hospital, Macau, Macau, Macao, SAR China
  • 6 Shenzhen University, Shenzhen, Guangdong Province, China
  • 7 Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China
  • 8 Guangzhou University, Guangzhou, Guangdong Province, China
  • 9 College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China

The final, formatted version of the article will be published soon.

    A UPLC-MS/MS method with one-step extraction and simultaneous determination of bongkrekic acid (BA) and dehydroacetic acid (DHA) in rice noodles has been established in this study for the first time. The extraction solvent consisted of a mixture of methanol, ammonia, and water. Electrospray ionization negative ion mode (ESI-) was used for multiple reaction detection and external standard method was used for quantitative determination. The method demonstrated a good linearity range of 0 to 50 ng/mL with a correlation coefficient (r) of 0.9998 for BA, and showed a linearity range of 0~500 ng/mL with r of 0.9993 for DHA. The limits of detection (LOD) were determined to be 0.1 μg/kg for BA and 0.6 μg/kg for DHA. Additionally, the method exhibited good recovery of 90.1%~105.4% for BA and 80.4%~102% for DHA.

    Keywords: Bongkrekic Acid, Dehydroacetic acid, UPLC-MS/MS, Burkholderia gladiolus, rice noodles

    Received: 15 Feb 2024; Accepted: 15 Nov 2024.

    Copyright: © 2024 Zhao, Ronggui, Song, Xi, lei, Weixian, Miaowen, Dabin, Xiaxia, Zhanguo, Xiao and Yong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Lv Ronggui, Shenzhen Hospital, Southern Medical University, Shenzhen, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.