AUTHOR=Haque Mst. Farjanamul , El-Nashar Heba A. S. , Akbor Md. Showkoth , Alfaifi Mohammed , Bappi Mehedi Hasan , Chowdhury Afsana Kabir , Hossain Muhammad Kamal , El-Shazly Mohamed , Albayouk Tala , Saleh Na’il , Islam Muhammad Torequl 

TITLE=Anti-inflammatory activity of d-pinitol possibly through inhibiting COX-2 enzyme: in vivo and in silico studies

JOURNAL=Frontiers in Chemistry

VOLUME=12

YEAR=2024

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2024.1366844

DOI=10.3389/fchem.2024.1366844

ISSN=2296-2646

ABSTRACT=<p><bold>Introduction:</bold> D-pinitol, a naturally occurring inositol, has diverse biological activities like antioxidant, antimicrobial and anticancer activities. This study aimed to evaluate anti-inflammatory effect of d-pinitol in a chick model. Additionally, <italic>in silico</italic> studies were performed to evaluate the molecular interactions with cyclooxygenase-2 (COX-2).</p><p><bold>Methods:</bold> The tested groups received d-pinitol (12.5, 25, and 50 mg/kg) and the standard drugs celecoxib and ketoprofen (42 mg/kg) via oral gavage prior to formalin injection. Then, the number of licks was counted for the first 10 min, and the paw edema diameter was measured at 60, 90, and 120 min.</p><p><bold>Results and Discussion:</bold> The d-pinitol groups significantly (<italic>p</italic> &lt; 0.05) reduced the number of paw licks and paw edema diameters, compared to negative control. When d-pinitol was combined with celecoxib, it reduced inflammatory parameters more effectively than the individual groups. The <italic>in silico</italic> study showed a promising binding capacity of d-pinitol with COX-2. Taken together, d-pinitol exerted anti-inflammatory effects in a dose-dependent manner, possibly through COX-2 interaction pathway.</p>