AUTHOR=Moon Hyungkyu , Sultana Tania , Lee JeongIk , Huh Jungrim , Lee Hae Dong , Choi Myung-Seok TITLE=Biomimetic lipid–fluorescein probe for cellular bioimaging JOURNAL=Frontiers in Chemistry VOLUME=11 YEAR=2023 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1151526 DOI=10.3389/fchem.2023.1151526 ISSN=2296-2646 ABSTRACT=

Fluorescence probe is one of the most powerful tools for cellular imaging. Here, three phospholipid-mimicking fluorescent probes (FP1–FP3) comprising fluorescein and two lipophilic groups of saturated and/or unsaturated C18 fatty acids were synthesized, and their optical properties were investigated. Like in biological phospholipids, the fluorescein group acts as a hydrophilic polar headgroup and the lipid groups act as hydrophobic non-polar tail groups. Laser confocal microscope images illustrated that FP3, which contains both saturated and unsaturated lipid tails, showed great uptake into the canine adipose-derived mesenchymal stem cells.