AUTHOR=Spratt Spencer J. , Mizuguchi Takaha , Akaboshi Hikaru , Kosakamoto Hina , Okada Rina , Obata Fumiaki , Ozeki Yasuyuki 

TITLE=Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering

JOURNAL=Frontiers in Chemistry

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1141920

DOI=10.3389/fchem.2023.1141920

ISSN=2296-2646

ABSTRACT=<p><bold>Introduction:</bold> Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging.</p><p><bold>Methods:</bold> Here, we used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporated into <italic>Drosophila</italic> tissues <italic>in vivo</italic>.</p><p><bold>Results</bold>: Our results demonstrate that SRS can detect a range of previously uncharacterized cell-to-cell differences in d-Met distribution within a tissue at the subcellular level.</p><p><bold>Discussion</bold>: These results demonstrate the potential of SRS microscopy for metabolic imaging of less abundant but important amino acids such as methionine in tissue.</p>