AUTHOR=Rodríguez-Moraga Nely , Ramos-Martín Francisco , Buchoux Sébastien , Rippa Sonia , D’Amelio Nicola , Sarazin Catherine 

TITLE=The effect of rhamnolipids on fungal membrane models as described by their interactions with phospholipids and sterols: An in silico study

JOURNAL=Frontiers in Chemistry

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1124129

DOI=10.3389/fchem.2023.1124129

ISSN=2296-2646

ABSTRACT=<p><bold>Introduction:</bold> Rhamnolipids (RLs) are secondary metabolites naturally produced by bacteria of the genera <italic>Pseudomonas</italic> and Burkholderia with biosurfactant properties. A specific interest raised from their potential as biocontrol agents for crop culture protection in regard to direct antifungal and elicitor activities. As for other amphiphilic compounds, a direct interaction with membrane lipids has been suggested as the key feature for the perception and subsequent activity of RLs.</p><p><bold>Methods:</bold> Molecular Dynamics (MD) simulations are used in this work to provide an atomistic description of their interactions with different membranous lipids and focusing on their antifungal properties.</p><p><bold>Results and discussion:</bold> Our results suggest the insertion of RLs into the modelled bilayers just below the plane drawn by lipid phosphate groups, a placement that is effective in promoting significant membrane fluidification of the hydrophobic core. This localization is promoted by the formation of ionic bonds between the carboxylate group of RLs and the amino group of the phosphatidylethanolamine (PE) or phosphatidylserine (PS) headgroups. Moreover, RL acyl chains adhere to the ergosterol structure, forming a significantly higher number of van der Waals contact with respect to what is observed for phospholipid acyl chains. All these interactions might be essential for the membranotropic-driven biological actions of RLs.</p>