AUTHOR=Lei Zimeng , Alwan Merim , Alamir Hassan Thoulfikar A. , Alkaaby Hussein Humedy Chlib , Farhan Sinan Subhi , Awadh Sura A. , Altimari Usama S. , Al-Baghdady Hawra’a Fadhel Abbas , Kadhim Athmar Ali , Qasim Maytham T. , Adhab Ali Hussein , Nekuei Abuzar TITLE=Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor JOURNAL=Frontiers in Chemistry VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.980162 DOI=10.3389/fchem.2022.980162 ISSN=2296-2646 ABSTRACT=

Detection of DNA molecules and possible chemotherapy-induced changes in its structure has been the goal of researchers using rapid, sensitive and inexpensive approaches. Therefore, the aim of this study was to fabricate a new electrochemical DNA biosensor using pencil graphite electrodes modified with polypyrrole/Ce doped hexagonal nickel oxide nanodisks or PP/Ce-doped H-NiO-ND composites for determination of Abemaciclib (AMC) and ds-DNA molecules. The DNA biosensor was prepared by immobilizing ds-DNA on the surface of PP/Ce-doped H-NiO-ND/PGE. Differential pulse voltammetry (DPV) was used to electrochemically detect AMC. The results elucidate the extremely high sensitivity of the ds-DNA/PP/Ce-doped H-NiO-ND/PGE biosensor to AMC, with a narrow detection limit of 2.7 nM and a lengthy linear range of 0.01–600.0 μM. The admirable performance of as-fabricated biosensor could be related to the active reaction sites and the unique electrochemical response related to the nanocomposites by enhancing ds-DNA stabilization and accelerating electron transfer on the surface of electrode.