AUTHOR=Peng Xingjia , Ang Song , Zhang Yizi , Fan Fenling , Wu Mengshuo , Liang Peiting , Wen Yan , Gan Lishe , Zhang Kun , Li Dongli , Yue Jianmin 

TITLE=Chemical Constituents With Antiproliferative Activity From Pogostemon cablin (Blanco) Benth.

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.938851

DOI=10.3389/fchem.2022.938851

ISSN=2296-2646

ABSTRACT=<p>Two new patchoulene sesquiterpenoid glycosides (<bold>1–2</bold>), a natural patchoulane-type sesquiterpenoid (<bold>3</bold>) and a natural cadinene-type sesquiterpenoid (<bold>4</bold>), were isolated from the aerial parts of <italic>Pogostemon cablin</italic> (Blanco) Benth., together with eleven known sesquiterpenoids (<bold>5</bold>–<bold>15</bold>) and eleven known flavonoids (<bold>16</bold>–<bold>26</bold>). Their chemical structures were elucidated on the basis of spectroscopic methods, including NMR, HRESIMS, IR, and CD spectroscopic data analysis, as well as chemical hydrolysis. The isolated compounds <bold>1</bold>–<bold>13</bold> and <bold>15</bold>–<bold>26</bold> were tested for inhibitory effects on the proliferation of HepG2 cancer cells. Among them, compounds <bold>17</bold> and <bold>19</bold> displayed anti-proliferative effects against HepG2 cells with IC<sub>50</sub> values of 25.59 and 2.30 μM, respectively. Furthermore, the flow cytometry analysis and Western blotting assays revealed that compound <bold>19</bold> significantly induced apoptosis of HepG2 cells by downregulating the ratio of Bcl-2/Bax and upregulating the expression of cleaved caspase-3 and cleaved caspase-9. Therefore, the potential pharmaceutical applications of <italic>P. cablin</italic> would be applied according to our study findings.</p>