AUTHOR=Wang Mengyun , Jiang Sai , Hussain Nusrat , Zafar Salman , Xie Qingling , Huang Feibing , Mao Linxi , Li Bin , Jian Yuqing , Wang Wei 

TITLE=Anti-RAFLS Triterpenoids and Hepatoprotective Lignans From the Leaves of Tujia Ethnomedicine Kadsura heteroclita (Xuetong)

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.878811

DOI=10.3389/fchem.2022.878811

ISSN=2296-2646

ABSTRACT=<p>A pair of 3,4-<italic>seco</italic>-cycloartane triterpenoid isomers with a rare peroxy bridge, namely, xuetonins A and B (<bold>1</bold> and <bold>2</bold>), four new lignans xuetonlignans A–D (<bold>3</bold>–<bold>6</bold>), a new sesquiterpene xuetonpene (<bold>7</bold>), and a new natural product xuetonin C (<bold>8</bold>), along with 43 known compounds, were obtained from the leaves of Tujia ethnomedicine, <italic>Kadsura heteroclita</italic>. Their structures and configurations were determined with the help of a combination of 1D- and 2D-NMR, HRESIMS spectra, electronic circular dichroism (ECD), and X-ray diffraction data. Compounds <bold>2</bold>, <bold>10</bold>, <bold>13</bold>–<bold>15</bold>, and <bold>17</bold>–<bold>19</bold> showed moderate-to-potent activity against rheumatoid arthritis fibroblast-like synoviocytes (RAFLS) with IC<sub>50</sub> values of 19.81 ± 0.26, 12.73 ± 0.29, 5.70 ± 0.24, 9.25 ± 0.79, 5.66 ± 0.52, 11.91 ± 0.44, 13.22 ± 0.27, and 15.94 ± 0.36 μM, respectively. Furthermore, compounds <bold>22</bold>, <bold>25</bold>, and <bold>31</bold> exhibited significant hepatoprotective effects against <italic>N</italic>-acetyl-<italic>p</italic>-aminophenol (APAP)–induced toxicity in HepG2 cells at 10 μM, and the cell viability increased by 12.93, 25.23, and 13.91%, respectively, compared with that in the model group (cf. bicyclol, 12.60%).</p>