AUTHOR=Huang Wei , Xu Yun , Sun Yimin 

TITLE=Functionalized Graphene Fiber Modified With MOF-Derived Rime-Like Hierarchical Nanozyme for Electrochemical Biosensing of H2O2 in Cancer Cells

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.873187

DOI=10.3389/fchem.2022.873187

ISSN=2296-2646

ABSTRACT=<p>The rational design and construction of high-performance flexible electrochemical sensors based on hierarchical nanostructure functionalized microelectrode systems are of vital importance for sensitive <italic>in situ</italic> and real-time detection of biomolecules released from living cells. Herein, we report a novel and facile strategy to synthesize a new kind of high-performance microelectrode functionalized by dual nanozyme composed of rime-like Cu<sub>2</sub>(OH)<sub>3</sub>NO<sub>3</sub> wrapped ZnO nanorods assembly [Cu<sub>2</sub>(OH)<sub>3</sub>NO<sub>3</sub>@ZnO], and explore its practical application in electrochemical detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) released from living cells. Benefiting from the merits of the unique hierarchical nanohybrid structure and high catalytic activities, the resultant Cu<sub>2</sub>(OH)<sub>3</sub>NO<sub>3</sub>@ZnO-modified AGF microelectrode shows remarkable electrochemical sensing performance towards H<sub>2</sub>O<sub>2</sub> with a low detection limit of 1 μM and a high sensitivity of 272 μA cm<sup>−2</sup> mM<sup>−1</sup>, as well as good anti-interference capability, long-term stability, and reproducibility. These properties enabled the proposed microelectrode-based electrochemical platform to be applied for <italic>in situ</italic> amperometric tracking of H<sub>2</sub>O<sub>2</sub> released from different types of human colon cells, thus demonstrating its great prospect as a sensitive cancer cell detection probe for the early diagnosis and management of various cancer diseases.</p>