AUTHOR=Yan Dongling , Liu Likun , Liu Xiangbao , Liu Qi , Hou Peng , Wang Hao , Xia Chunhui , Li Gang , Ma Chunhui , Chen Song TITLE=Simultaneous Discrimination of Cys/Hcy and GSH With Simple Fluorescent Probe Under a Single-Wavelength Excitation and its Application in Living Cells, Tumor Tissues, and Zebrafish JOURNAL=Frontiers in Chemistry VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.856994 DOI=10.3389/fchem.2022.856994 ISSN=2296-2646 ABSTRACT=
Owing to the important physiological sits of biothiols (Cys, Hcy, and GSH), developing accurate detection methods capable of qualitative and quantitative analysis of biothiols in living systems is needed for understanding the biological profile of biothiols. In this work, we have designed and synthesized a 4′-hydroxy-[1,1′-biphenyl]-4-carbonitrile modified with NBD group-based fluorescent probe, BPN-NBD, for sensitive detection of Cys/Hcy and GSH by dual emission signals via a single-wavelength excitation. BPN