AUTHOR=Bilal Bushra , Niazi Rimsha , Nadeem Sohail , Farid Muhammad Asim , Nazir Muhammad Shahid , Akhter Toheed , Javed Mohsin , Mohyuddin Ayesha , Rauf Abdul , Ali Zulfiqar , Naqvi Syed Ali Raza , Muhammad Nawshad , Elkaeed Eslam B. , Ibrahium Hala A. , Awwad Nasser S. , Hassan Sadaf Ul 

TITLE=Fabrication of Guided Tissue Regeneration Membrane Using Lignin-Mediated ZnO Nanoparticles in Biopolymer Matrix for Antimicrobial Activity

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.837858

DOI=10.3389/fchem.2022.837858

ISSN=2296-2646

ABSTRACT=<p>Periodontal disease is a common complication, and conventional periodontal surgery can lead to severe bleeding. Different membranes have been used for periodontal treatment with limitations, such as improper biodegradation, poor mechanical property, and no effective hemostatic property. Guided tissue regeneration (GTR) membranes favoring periodontal regeneration were prepared to overcome these shortcomings. The mucilage of the chia seed was extracted and utilized to prepare the guided tissue regeneration (GTR) membrane. Lignin having antibacterial properties was used to synthesize lignin-mediated ZnO nanoparticles (∼Lignin@ZnO) followed by characterization with analytical techniques like Fourier-transform infrared spectroscopy (FTIR), UV–visible spectroscopy, and scanning electron microscope (SEM). To fabricate the GTR membrane, extracted mucilage, Lignin@ZnO, and polyvinyl alcohol (PVA) were mixed in different ratios to obtain a thin film. The fabricated GTR membrane was evaluated using a dynamic fatigue analyzer for mechanical properties. Appropriate degradation rates were approved by degradability analysis in water for different intervals of time. The fabricated GTR membrane showed excellent antibacterial properties against <italic>Staphylococcus aureus</italic> (<italic>S. aureus</italic>) and <italic>Escherichia coli</italic> (<italic>E. coli</italic>) bacterial species.</p>