AUTHOR=Yin Zhen-Hua , Liu Xiao-Peng , Wang Jin-Mei , Xi Xue-Feng , Zhang Yan , Zhao Rui-Lin , Kang Wen-Yi 

TITLE=Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.825127

DOI=10.3389/fchem.2022.825127

ISSN=2296-2646

ABSTRACT=<p><italic>Pleurotus placentodes</italic>, a fungus, belongs to the Pleurotaceae family. The aim of the present study was to characterize the structure of a novel polysaccharide from fruiting bodies of <italic>P. placentodes</italic> (PPp-W) and evaluate its anticoagulant activity <italic>in vitro</italic>. The high-performance liquid chromatography and GC–MS analysis indicated that PPp-W with a molecular weight of 27.4 kDa was mainly composed of mannose (17.56%), glucose (6.37%), galactose (44.89%), and fucose (1.22%) with a certain amount of 3-<italic>O</italic>-methyled galactose. SEM, XRD, and AFM combined with Congo red test revealed that PPp-W was an irregular curly sheet with triple-helix conformation. The FT-IR, methylation, and nuclear magnetic resonance analysis indicated that PPp-W contained→6)-α-D-Gal<italic>p</italic>-(1→, →6)-3-<italic>O</italic>-Me-α-D-Gal<italic>p</italic>-(1→and →2, 6)-α-D-Gal<italic>p</italic>-(1→ as main chain, partially substituted at <italic>O</italic>-2 and <italic>O</italic>-6 by non-reducing ends of β<italic>-</italic>D<italic>-</italic>Man<italic>p</italic>-(1→ and β<italic>-</italic>L-Fuc<italic>p</italic>-(1→ with a small amount of α-1,3-linked-Glc<italic>p</italic> in backbone. PPp-W could significantly prolong APTT (12.9 ± 0.42 s, <italic>p</italic> &lt; 0.001) and thrombin time (39.9 ± 0.28 s, <italic>p</italic> &lt; 0.01) compared with the control group (11.45 ± 0.071 s and 38.05 ± 0.21 s), which showed that PPp-W had anticoagulant activity. These studies suggested that PPp-W was a 3-<italic>O</italic>-methylated heteroglycan and might be suitable for functional foods and natural drugs as an anticoagulant ingredient, which provided a basis for the application of polysaccharides from <italic>P. placentodes</italic>.</p>