AUTHOR=Abbasi Mahmoud , Alsaikhan Fahad , Obaid Rasha Fadhel , Jahani Shohreh , Biroudian Saeed , Oveisee Maziar , Arab Mohammad Reza , Aramesh-Boroujeni Zahra , Foroughi Mohammad Mehdi 

TITLE=Development of the DNA-based voltammetric biosensor for detection of vincristine as anticancer drug

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.1060706

DOI=10.3389/fchem.2022.1060706

ISSN=2296-2646

ABSTRACT=<p>In the article presented herein, a deoxyribonucleic acid (DNA) biosensor is introduced for Vincristine determination in pharmaceutical preparations based on the modification of screen printed electrode (SPE) with double-stranded DNA (ds-DNA), polypyrrole (PP), peony-like CuO:Tb<sup>3+</sup> nanostructure (P-L CuO:Tb<sup>3+</sup> NS). The developed sensor indicated a wide linear response to Vincristine concentration ranged from 1.0 nM to 400.0 μM with a limit of detection as low as .21 nM. The intercalation of Vincristine with DNA guanine led to the response. The optimized parameters for the biosensor performance were ds-DNA/Vincristine interaction time, DNA concentration and type of buffer solution. The docking investigation confirm the minor groove interaction between guanine base at surface of or ds-DNA/PP/P-L CuO:Tb<sup>3+</sup> NS/SPE and Vincristine. The proposed sensor could successfully determine Vincristine in Vincristine injections and biological fluids, with acceptable obtains.</p>