AUTHOR=Permatasari Happy Kurnia , Barbara Ulfa Ektina Naura , Adyana Daud Vanessa Pradna , Sulistomo Hikmawan Wahyu , Nurkolis Fahrul 

TITLE=Caulerpa racemosa extract inhibits HeLa cancer cells migration by altering expression of epithelial-mesenchymal transition proteins

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.1052238

DOI=10.3389/fchem.2022.1052238

ISSN=2296-2646

ABSTRACT=<p><bold>Introduction:</bold> Cervical cancer is caused by persistent infections of human papillomavirus types 16 and 18. Also, it is classified as a malignancy since it is able to spread itself to other sites and form a metastasis. Lymph nodes metastasis is an important factor related to cervical cancer survival. The previous study reported that <italic>Caulerpa racemosa</italic> has an anti-cancer effect by inducing apoptosis by inhibiting p53 protein degradation in HeLa cancer cells. In this study, we conducted a follow-up test to determine the anticancer effect of <italic>Caulerpa racemosa</italic> as an antimetastatic agent on HeLa cancer cells.</p><p><bold>Methods:</bold> A true experimental study with a post-test-controlled group design was carried out on four groups of HeLa cell cultures by presenting different concentrations of <italic>Caulerpa racemosa</italic> extract. Moreover, to identify the antimetastatic effect, HeLa cells treated with <italic>Caulerpa racemosa</italic> extract were subjected to the woud healing scratch test and immunofluorescence staining assays. Data analysis was gained with qualitative and quantitative approaches. Quantitative methods such as One-way analysis of variance, Tukey’s multiple comparison test, and Pearson’s correlation were conducted.</p><p><bold>Result:</bold> We found that <italic>Caulerpa racemosa</italic> significantly inhibit HeLa cells wound healing migration. We also demonstrated the effect of <italic>Caulerpa racemosa</italic> in downregulating Snail and Vimentin protein expression and upregulating E-Cadherin protein expression.</p><p><bold>Conclusion:</bold><italic>Caulerpa racemosa</italic> extract inhibits HeLa cancer cells migration by altering important regulator proteins expressions of epithelial-mesenchymal transition pathways.</p>