AUTHOR=Ma Le , Zong Jiajia , Xun Xiaowei , Hu Xiaoming , Chen Zejing , Zhang Quanchao , Peng Mengxia , Song Botao , Ao Haiyong 

TITLE=Fabrication of gentamicin loaded Col-I/HA multilayers modified titanium coatings for prevention of implant infection

JOURNAL=Frontiers in Chemistry

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.1019332

DOI=10.3389/fchem.2022.1019332

ISSN=2296-2646

ABSTRACT=<p>In this study, gentamicin loaded collagen I/hyaluronic acid multilayers modified titanium coating (TC-AA(C/H)<sub>6</sub>-G) was fabricated <italic>via</italic> a layer-by-layer (LBL) covalent immobilization method. The drug releasing properties of collagen I/Hyaluronic acid (Col-I/HA) multilayers and the effect of loaded gentamicin on the antibacterial properties and cytocompatibility of modified TC were investigated. The gentamicin release assay indicated that the Col-I/HA multilayers modified TC exhibited agreeable drug-loading amount (537.22 ± 29.66 µg of gentamicin) and controlled-release performance (240 h of sustained release time). TC-AA(C/H)<sub>6</sub>-G revealed satisfactory antibacterial activity and inhibited the colonization and biofilm formation of <italic>S. aureus</italic>. Fortunately, the functions of hMSCs on TC-AA(C/H)<sub>6</sub>-G did not affected by the loaded gentamicin, and TC-AA(C/H)<sub>6</sub>-G could improve the adhesion, proliferation and osteogenic differentiation of cells, as well as TC-AA(C/H)<sub>6</sub>. <italic>In vivo</italic> animal study indicated that TC-AA(C/H)<sub>6</sub>-G could effectively control intramedullary cavity infection caused by <italic>S. aureus</italic> and prevent bone destruction.</p>